LOCUS       sequence-286122-        1265 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3193
FEATURES             Location/Qualifiers
     source          1..1265
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1097..1121)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1129..1170
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc agtccaagcg ggattgtgag
       61 ctgtggtgtg agagggtgaa tccagagaac aaggcggcgc tggaggcgtg ggtcagggag
      121 acaggcatcc gcctggtgca ggtgaacggg cagaggaagt atggcgggcc acccccaggc
      181 tgggtgggca gcccgccgcc agctgggtca gaggtgttca tcgggcggct gcctcaggac
      241 gtgtacgagc accagcttat cccgctgttc cagcgcgtgg gccgcctcta cgagttccgc
      301 ctgatgatga ccttcagcgg cctgaaccgc ggcttcgcct atgcccgcta cagctcgagg
      361 cgcggcgcgc aggccgccat cgccacgctg cacaaccatc cgctgcggcc gtcctgcccg
      421 ctgctcgtgt gccgcagcac cgagaagtgt gagctgagcg ttgacggcct gccgccgaat
      481 ctgacccgca gcgcgctgct gctcgcgctg cagccgctgg gtcccggctt gcaggaggcg
      541 cggctgctgc ccagccccgg accggcgccc gggcagatcg ctctgctcaa attcagctcg
      601 caccgggccg ctgccatggc caaaaaggcc ctggtggaag ggcagtcaca cctctgtgga
      661 gagcaggtgg ctgtggagtg gctcaagcca gacctgaagc agcgacttcg ccagcagctt
      721 gtgggtccct tcttgcggtc cccacagcca gagggcagcc agttggcttt ggcaagggac
      781 aagttagggt tccaaggggc tcgggctacc ctgcagttgc tgtgccaacg aatgaagctg
      841 ggcagccctg tgttcctcac caagtgtttg ggcataggac ctgctggctg gcaccgcttc
      901 tggtaccagg tggtgattcc tgggcatccg gtgcccttca gcggcctcat ctgggttgtg
      961 ctgaccctag atggccggga tgggcatgag gtggccaagg atgctgtgtc tgtacggctg
     1021 ctgcaggcac tcagtgagtc tggggccaac ctcctgtggt ctgctggggc tgaggcaggt
     1081 accatggtta aacagtgccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     1141 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1201 atttcgattt cttggcttta tatatcttgt ggaaaggacg attgatgcac atagcacggg
     1261 tacct
//
