LOCUS       sequence-286125-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3194
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(911..935)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             943..984
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg aggcgcccgc cagcgcgcag
       61 accccgcacc cgcacgagcc catcagcttc ggcatcgacc agatccttaa cagcccggac
      121 caggacagcg cacccgcccc gcggggcccc gacggcgcca gctacctggg agggcccccc
      181 gggggccgtc cgggcgccac atacccgtct ctgcccgcct cctttgcggg cctcggcgcg
      241 cccttcgagg acgcgggatc ttacagtgtg aacctgagcc tagcgcccgc aggcgtgatc
      301 cgggtgccgg cgcacaggcc gctgcccggg gccgtgccac cgcctctgcc aagcgcgcta
      361 cccgccatgc cctccgtgcc cacggtctcc agccttggcg gtctcaattt cccctggatg
      421 gagagcagcc gccgcttcgt gaaagaccgc ttcacagcgg cggccgcact cacgcccttc
      481 accgtgaccc ggcgcatcgg ccacccctac cagaaccgga cgccgcccaa gcgtaagaag
      541 ccgcgcacgt ccttttcccg ggtgcagatc tgcgagctgg aaaagcgctt ccatcgccag
      601 aagtacctgg cctctgccga gagggcggcg ctcgccaagt ccctcaaaat gacggacgcg
      661 caggtcaaga cctggttcca aaaccggagg accaagtggc ggcggcagac ggcggaggag
      721 cgggaggcgg agcggcagca ggcgagccgg ctcatgctgc agctgcaaca cgacgccttc
      781 caaaagagcc tcaacgactc catccagcct gacccgctct gtctgcacaa ctcgtcactc
      841 tttgctctgc agaatctgca gccctgggag gaggatagtt ccaaggttcc cgctgtcacc
      901 tccctggtgt gcccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
      961 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1021 atttcttggc tttatatatc ttgtggaaag gacgactgag aatcgagcat cctcaccca
//
