LOCUS       sequence-286128-        1604 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3195
FEATURES             Location/Qualifiers
     source          1..1604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             409..438
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             412..441
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     protein_bind    complement(1436..1460)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1468..1509
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgaacgcgca gctgaccatg
       61 gaagcgatcg gcgagctgca cggggtgagc catgagccgg tgcccgcccc tgccgacctg
      121 ctgggcggca gcccccacgc gcgcagctcc gtggcgcacc gcggcagcca cctgcccccc
      181 gcgcacccgc gctccatggg catggcgtcc ctgctggacg gcggcagcgg cggcggagat
      241 taccaccacc accaccgggc ccctgagcac agcctggccg gccccctgca tcccaccatg
      301 accatggcct gcgagactcc cccaggtatg agcatgccca ccacctacac caccttgacc
      361 cctctgcagc cgctgcctcc catctccaca gtctcggaca agttccccca ccatcaccac
      421 caccaccatc accaccacca cccgcaccac caccagcgcc tggcgggcaa cgtgagcggt
      481 agcttcacgc tcatgcggga tgagcgcggg ctggcctcca tgaataacct ctataccccc
      541 taccacaagg acgtggccgg catgggccag agcctctcgc ccctctccag ctccggtctg
      601 ggcagcatcc acaactccca gcaagggctc ccccactatg cccacccggg ggccgccatg
      661 cccaccgaca agatgctcac ccccaacggc ttcgaagccc accacccggc catgctcggc
      721 cgccacgggg agcagcacct cacgcccacc tcggccggca tggtgcccat caacggcctt
      781 cctccgcacc atccccacgc ccacctgaac gcccagggcc acgggcaact cctgggcaca
      841 gcccgggagc ccaacccttc ggtgaccggc gcgcaggtca gcaatggaag taattcaggg
      901 cagatggaag agatcaatac caaagaggtg gcgcagcgta tcaccaccga gctcaagcgc
      961 tacagcatcc cacaggccat cttcgcgcag agggtgctct gccgctccca ggggaccctc
     1021 tcggacctgc tgcgcaaccc caaaccctgg agcaaactca aatccggccg ggagaccttc
     1081 cggaggatgt ggaagtggct gcaggagccg gagttccagc gcatgtccgc gctccgctta
     1141 gcagcatgca aaaggaaaga acaagaacat gggaaggata gaggcaacac acccaaaaag
     1201 cccaggttgg tcttcacaga tgtccagcgt cgaactctac atgcaatatt caaggaaaat
     1261 aagcgtccat ccaaagaatt gcaaatcacc atttcccagc agctggggtt ggagctgagc
     1321 actgtcagca acttcttcat gaacgcaaga aggaggagtc tggacaagtg gcaggacgag
     1381 ggcagctcca attcaggcaa ctcatcttct tcatcaagca cttgtaccaa agcattgcca
     1441 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
     1501 gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1561 atatcttgtg gaaaggacga ttggtataag caggcaatcc gtta
//
