LOCUS       sequence-286131-        1550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3196
FEATURES             Location/Qualifiers
     source          1..1550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1382..1406)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1414..1455
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgctcctgga cgccggcccc
       61 cagtacccag cgatcggcgt gaccaccttt ggcgcgtccc gccaccactc cgcgggcgac
      121 gtggccgaac gagacgtggg cctgggcatc aacccgttcg ccgacggcat gggcgccttc
      181 aagctcaacc ccagttcgca cgagctggct tcggccggcc agacggcctt cacgtcgcag
      241 gcgccaggct acgcggctgc tgcggccctg ggccatcacc atcacccggg ccacgtcggc
      301 tcctattcca gcgcagcctt caactccacg cgggactttc tgttccgcaa ccggggtttt
      361 ggcgacgcgg cggcggcagc cagcgcacag cacagcctct ttgctgcatc ggccgggggc
      421 ttcgggggcc cacacggcca cacggacgcc gcgggccacc tcctcttccc cgggcttcac
      481 gagcaggctg ccggccacgc gtcgcctaac gtggtcaacg ggcagatgag gctcggcttc
      541 tcgggggaca tgtacccgcg accggagcag tacggccagg tgaccagccc gcgttcggag
      601 cactatgctg cgccgcagct gcacggctac gggcccatga acgtgaacat ggccgcgcat
      661 cacggcgccg gcgccttctt ccgctacatg cgccaaccca tcaagcaaga gctcatctgc
      721 aagtggatcg agcccgagca gctggccaac cccaaaaagt cgtgcaacaa aactttcagc
      781 accatgcacg agctagttac gcacgtcacc gtggagcacg taggtggccc ggagcagagt
      841 aatcacatct gcttctggga ggagtgtccg cgcgagggca agcccttcaa agccaaatac
      901 aaactggtta accacatccg cgtgcacacg ggcgagaagc cctttccctg ccccttccct
      961 ggctgtggca aggtcttcgc gcgctccgag aatttaaaga tccacaaaag gacgcacaca
     1021 ggggagaagc ccttcaagtg cgagtttgag ggctgtgacc ggcgcttcgc taacagcagc
     1081 gaccgcaaga agcacatgca cgtgcacacg agcgacaagc cctatctttg caagatgtgc
     1141 gacaagtcct acacgcatcc cagttcgctg cgcaaacaca tgaaggtcca cgaatcctcc
     1201 tcgcagggct cgcagccttc gccggccgcc agctctggct acgaatcctc cacgcctccc
     1261 accatcgtgt ctccctccac agacaacccg accacaagct ccttatcgcc ctcctcctcc
     1321 gcagtccacc acacagccgg ccacagtgcg ctctcttcca attttaacga atggtacgtt
     1381 ttgccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     1441 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1501 ctttatatat cttgtggaaa ggacgagttt gcaaccaatc cccctattga
//
