LOCUS       sequence-286134-        2588 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3197
FEATURES             Location/Qualifiers
     source          1..2588
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2420..2444)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2452..2493
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cgggctggat ccaggcccag
       61 cagctgcagg gagacgcgct gcgccagatg caggtgctgt acggccagca cttccccatc
      121 gaggtccggc actacttggc ccagtggatt gagagccagc catgggatgc cattgacttg
      181 gacaatcccc aggacagagc ccaagccacc cagctcctgg agggcctggt gcaggagctg
      241 cagaagaagg cggagcacca ggtgggggaa gatgggtttt tactgaagat caagctgggg
      301 cactacgcca cgcagctcca gaaaacatat gaccgctgcc ccctggagct ggtccgctgc
      361 atccggcaca ttctgtacaa tgaacagagg ctggtccgag aagccaacaa ttgcagctct
      421 ccggctggga tcctggttga cgccatgtcc cagaagcacc ttcagatcaa ccagacattt
      481 gaggagctgc gactggtcac gcaggacaca gagaatgagc tgaagaaact gcagcagact
      541 caggagtact tcatcatcca gtaccaggag agcctgagga tccaagctca gtttgcccag
      601 ctggcccagc tgagccccca ggagcgtctg agccgggaga cggccctcca gcagaagcag
      661 gtgtctctgg aggcctggtt gcagcgtgag gcacagacac tgcagcagta ccgcgtggag
      721 ctggccgaga agcaccagaa gaccctgcag ctgctgcgga agcagcagac catcatcctg
      781 gatgacgagc tgatccagtg gaagcggcgg cagcagctgg ccgggaacgg cgggcccccc
      841 gagggcagcc tggacgtgct acagtcctgg tgtgagaagt tggccgagat catctggcag
      901 aaccggcagc agatccgcag ggctgagcac ctctgccagc agctgcccat ccccggccca
      961 gtggaggaga tgctggccga ggtcaacgcc accatcacgg acattatctc agccctggtg
     1021 accagcacat tcatcattga gaagcagcct cctcaggtcc tgaagaccca gaccaagttt
     1081 gcagccaccg tacgcctgct ggtgggcggg aagctgaacg tgcacatgaa tcccccccag
     1141 gtgaaggcca ccatcatcag tgagcagcag gccaagtctc tgcttaaaaa tgagaacacc
     1201 cgcaacgagt gcagtggtga gatcctgaac aactgctgcg tgatggagta ccaccaagcc
     1261 acgggcaccc tcagtgccca cttcaggaac atgtcactga agaggatcaa gcgtgctgac
     1321 cggcggggtg cagagtccgt gacagaggag aagttcacag tcctgtttga gtctcagttc
     1381 agtgttggca gcaatgagct tgtgttccag gtgaagactc tgtccctacc tgtggttgtc
     1441 atcgtccacg gcagccagga ccacaatgcc acggctactg tgctgtggga caatgccttt
     1501 gctgagccgg gcagggtgcc atttgccgtg cctgacaaag tgctgtggcc gcagctgtgt
     1561 gaggcgctca acatgaaatt caaggccgaa gtgcagagca accggggcct gaccaaggag
     1621 aacctcgtgt tcctggcgca gaaactgttc aacaacagca gcagccacct ggaggactac
     1681 agtggcctgt ccgtgtcctg gtcccagttc aacagggaga acttgccggg ctggaactac
     1741 accttctggc agtggtttga cggggtgatg gaggtgttga agaagcacca caagccccac
     1801 tggaatgatg gggccatcct aggttttgtg aataagcaac aggcccacga cctgctcatc
     1861 aacaagcccg acgggacctt cttgttgcgc tttagtgact cagaaatcgg gggcatcacc
     1921 atcgcctgga agtttgattc cccggaacgc aacctgtgga acctgaaacc attcaccacg
     1981 cgggatttct ccatcaggtc cctggctgac cggctggggg acctgagcta tctcatctat
     2041 gtgtttcctg accgccccaa ggatgaggtc ttctccaagt actacactcc tgtgctggct
     2101 aaagctgttg atggatatgt gaaaccacag atcaagcaag tggtccctga gtttgtgaat
     2161 gcatctgcag atgctggggg cagcagcgcc acgtacatgg accaggcccc ctccccagct
     2221 gtgtgccccc aggctcccta taacatgtac ccacagaacc ctgaccatgt actcgatcag
     2281 gatggagaat tcgacctgga tgagaccatg gatgtggcca ggcacgtgga ggaactctta
     2341 cgccgaccaa tggacagtct tgactcccgc ctctcgcccc ctgccggtct tttcacctct
     2401 gccagaggct ccctctcatg cccaactttc ttgtacaaag tggttgatat cggtaagcct
     2461 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
     2521 agtatttcga tttcttggct ttatatatct tgtggaaagg acgagcagct agctcaggac
     2581 tttaaccg
//
