LOCUS       sequence-286137-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3198
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(863..887)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             895..936
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttgacatgc ccaaagtctt cctggtgaag
       61 aggaggagcc tgggggtctc ggtccgcagc tgggatgagc tcccggatga gaaaagggca
      121 gacacctaca tcccagtggg cctaggccgc ctgctccacg acccccccga ggactgccgc
      181 agcgacggcg gcagcagcag cggcagcggc agcagcagcg cgggggagcc tggaggagca
      241 gagagcagct cgtccccgca cgcccccgag agcgaaaccc ccgagcccgg cgacgccgag
      301 ggccccgatg gacacctggc gaccaagcag cgcccggtcg ccagatcgaa aatcaagttc
      361 accacaggca cgtgcagcga ctcggtggtt cacagctgtg acctgtgtgg caagggcttc
      421 cgtctgcagc gcatgctgaa ccgtcacctc aagtgccaca accaggtgaa aagacacctg
      481 tgcaccttct gcggcaaggg cttcaacgac accttcgacc tgaagaggca cgtccgcaca
      541 cacacaggca ttcgtcccta caaatgcaac gtctgcaata aagccttcac ccagcgctgc
      601 tctctggagt cccacctgaa gaaaatccat ggggtgcagc agcagtatgc ctataagcag
      661 cggcgggaca agctctacgt ctgcgaggat tgcggctaca cgggccccac ccaggaggac
      721 ctgtacctgc acgtgaacag tgcccatccg ggcagctcgt ttctcaaaaa gacatctaaa
      781 aaactggcag cccttctgca gggcaagctg acatccgcac accaggagaa taccagcctg
      841 agtgaggagg aggagaggaa gtgctcaact ttcttgtaca aagtggttga tatcggtaag
      901 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
      961 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagca aggtggtgtt
     1021 tacctgcatc a
//
