LOCUS       sequence-286143-        1493 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3200
FEATURES             Location/Qualifiers
     source          1..1493
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1109..1118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1325..1349)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1357..1398
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg catcgaacag catcttcgac
       61 tccttcccga cctactcgcc gaccttcatc cgcgacccaa gcaccagccg ccgcttcaca
      121 cctccctccc cggccttccc ctgcggcggc ggcggcggca agatgggcga gaacagcggc
      181 gcgctgagcg cgcaggcggc cgtggggccc ggagggcgcg cccggcccga ggtgcgctcg
      241 atggtggacg tgctggcgga ccacgcaggc gagctcgtgc gcaccgacag ccccaacttc
      301 ctctgctccg tgctgccctc gcactggcgc tgcaacaaga cgctgcccgt cgccttcaag
      361 gtggtggcat tgggggacgt gccggatggt acggtggtga ctgtgatggc aggcaatgac
      421 gagaactact ccgctgagct gcgcaatgcc tcggccgtca tgaagaacca ggtggccagg
      481 ttcaacgacc ttcgcttcgt gggccgcagt gggcgaggga agagtttcac cctgaccatc
      541 actgtgttca ccaaccccac ccaagtggcg acctaccacc gagccatcaa ggtgaccgtg
      601 gacggacccc gggagcccag acggcaccgg cagaagctgg aggaccagac caagccgttc
      661 cctgaccgct ttggggacct ggaacggctg cgcatgcggg tgacaccgag cacacccagc
      721 ccccgaggct cactcagcac cacaagccac ttcagcagcc agccccagac cccaatccaa
      781 ggcacctcgg aactgaaccc attctccgac ccccgccagt ttgaccgctc cttccccacg
      841 ctgccaaccc tcacggagag ccgcttccca gaccccagga tgcattatcc cggggccatg
      901 tcagctgcct tcccctacag cgccacgccc tcgggcacga gcatcagcag cctcagcgtg
      961 gcgggcatgc cggccaccag ccgcttccac catacctacc tcccgccacc ctacccgggg
     1021 gccccgcaga accagagcgg gcccttccag gccaacccgt ccccctacca cctctactac
     1081 gggacatcct ctggctccta ccagttctcc atggtggccg gcagcagcag tgggggcgac
     1141 cgctcaccta cccgcatgct ggcctcttgc accagcagcg ctgcctctgt cgccgccggc
     1201 aacctcatga accccagcct gggcggccag agtgatggcg tggaggccga cggcagccac
     1261 agcaactcac ccacggccct gagcacgcca ggccgcatgg atgaggccgt gtggcggccc
     1321 tactgcccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     1381 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1441 tggctttata tatcttgtgg aaaggacgac actgaactgg tggtgttcga cgc
//
