LOCUS       sequence-286146-        1505 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3201
FEATURES             Location/Qualifiers
     source          1..1505
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1337..1361)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1369..1410
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgccccattc tcctctgatc
       61 tccattcctc atgtgtggtg tcacccagaa gaggaggaaa gaatgcatga tgaacttcta
      121 caagcagtat ccaaggggcc ggtgatgttc agggatgttt ccatagactt ctctcaagag
      181 gaatgggaat gcctggacgc tgatcagatg aatttataca aagaagtgat gttggagaat
      241 ttcagcaacc tggtttcagt gggactttcc aattctaagc cagctgtgat ctccttattg
      301 gaacaaggaa aagagccctg gatggttgat agagagctga ctagaggcct gtgttcagat
      361 ctggaatcaa tgtgtgagac caaaatatta tctctaaaga agagacattt cagtcaagta
      421 ataattaccc gtgaagacat gtctactttt attcagccca catttcttat tccacctcaa
      481 aaaactatga gtgaagagaa accatgggaa tgtaagatat gtggaaagac ctttaatcaa
      541 aactcacaat ttatccaaca tcagagaatt cattttggtg aaaaacacta tgaatctaag
      601 gagtatggga agtcctttag tcgtggctca ctcgttactc gacatcagag gattcacact
      661 ggtaaaaaac cctatgaatg taaggaatgt ggcaaggctt ttagttgtag ttcatatttt
      721 tctcaacatc agaggattca cactggtgag aaaccctatg aatgtaagga atgtggaaaa
      781 gcctttaagt attgctcaaa ccttaatgat catcagagaa ttcacactgg tgagaaaccc
      841 tatgaatgta aagtatgtgg aaaagccttt actaaaagtt cacaactttt tctacatctg
      901 agaattcata ctggtgagaa accttatgaa tgtaaagaat gtgggaaagc ctttactcaa
      961 cactcaaggc ttattcagca tcagagaatg catactggtg agaaacctta tgaatgtaag
     1021 cagtgtggga aggcctttaa tagtgcctca acacttacta accatcacag aattcatgct
     1081 ggtgagaagc tctatgaatg tgaagaatgt agaaaggcct ttattcagag ctcagaactt
     1141 attcaacatc agagaatcca tacagatgaa aaaccatatg aatgtaatga atgtgggaag
     1201 gcctttaata aaggctcaaa tcttactcga catcagagaa ttcacactgg tgagaaaccc
     1261 tatgactgta aggaatgtgg aaaggctttt ggtagtcgct ctgacctcat tcgccatgag
     1321 ggaattcata ctggtttgcc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     1381 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1441 atttcgattt cttggcttta tatatcttgt ggaaaggacg acctagttta atctgaaaga
     1501 ctgcg
//
