LOCUS       sequence-286149-        2162 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3202
FEATURES             Location/Qualifiers
     source          1..2162
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1994..2018)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2026..2067
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgttacgtga ggaagccacc
       61 aagaagagca aagaaaagga gccagggatg gctcttcctc agggacgctt gactttcagg
      121 gatgtggcta tagagttctc tttggaggag tggaaatgcc tgaaccctgc acagagggct
      181 ttatacaggg ctgtgatgtt ggagaactac aggaacctgg agtttgtgga tagctcttta
      241 aaatccatga tggagttctc atcaaccagg cacagtatta caggagaagt gatccacaca
      301 gggacgttgc aaagacataa aagtcatcac attggagatt tttgcttccc agaaatgaag
      361 aaagatattc atcactttga gtttcagtgg caagaagttg aaagaaatgg ccatgaagca
      421 cccatgacaa aaatcaaaaa gttgactggt agtacagacc gaagtgatca caggcatgct
      481 ggaaacaagc ctattaaaga tcagcttgga ttaagctttc attcgcatct gcctgaactc
      541 cacatgtttc agactaaagg gaaaattagt aaccaattgg acaagtctat cggtgcttcc
      601 tcagcttcag aatcccaaag aatttcttgt aggctcaaaa ctcatatttc taataagtat
      661 gggaagaatt tcctccattc ttcattcaca caaatacagg aaatatgcat gagagaaaaa
      721 ccttgccaaa gtaatgagtg tggcaaagcc tttaattata gctcactctt aaggagacac
      781 cacataaccc attcaagaga gagagaatat aaatgtgatg tatgtggcaa gatctttaat
      841 cagaagcaat acattgtata tcatcataga tgtcacactg gtgagaaaac ttacaagtgt
      901 aatgagtgtg ggaagacctt cactcagatg tcatcccttg tatgccatcg tagacttcat
      961 actggagaga aaccttacaa gtgtaatgag tgtggcaaga ccttcagtga gaagtcatcc
     1021 cttagatgcc atcgtagact tcatactgga gagaaacctt acaagtgtaa tgagtgtggc
     1081 aagacttttg gtcgaaattc agcccttgta attcataagg caattcatac tggagagaaa
     1141 ccttacaagt gtaatgagtg tggcaagacc ttcagtcaga aatcatccct tcaatgccat
     1201 catatacttc acactggaga gaaaccttac aaatgtgaag aatgtgacaa tgtttacatt
     1261 cgcagatcac accttgaaag acataggaag attcatactg gagagggatc atacaaatgt
     1321 aaggtttgtg acaaggtttt ccggagtgat tcataccttg cagaacatca gagagttcat
     1381 actggagaga aaccatacaa gtgtaataaa tgtggcagga gtttcagtcg gaagtcatcc
     1441 cttcaatacc atcatacact tcacactgga gagaaacctt acacatgtaa tgaatgtggc
     1501 aaggttttta gtcgaagaga aaaccttgca cgtcatcata gacttcatgc tggagagaaa
     1561 ccttacaaat gtgaagaatg tgacaaagtt ttcagtcgca gatcacacct tgaaagacat
     1621 aggagaattc atactgggga aaaaccatac aaatgtaagg tttgtgacaa ggctttccgg
     1681 agtgattcat gccttgcaaa ccatacgaga gttcatactg gagagaaacc ttacaagtgt
     1741 aataaatgtg cgaaggtttt taatcaaaaa ggaatccttg cacaacatca gagagttcat
     1801 actggagaga aaccttacaa gtgtaatgaa tgtggcaagg tttttaatca aaaagcaagc
     1861 cttgcaaaac atcagagagt tcatactgca gagaaacctt acaagtgtaa tgagtgtggc
     1921 aaagccttta ctggacagtc aacacttatt caccatcaag caatccatgg gtgtagggaa
     1981 actttacaaa tgttgccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
     2041 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     2101 tcgatttctt ggctttatat atcttgtgga aaggacgacg tgccttgtcg aagccacgtt
     2161 aa
//
