LOCUS       sequence-286152-        2216 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3203
FEATURES             Location/Qualifiers
     source          1..2216
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2048..2072)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2080..2121
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cagaggtggt ggctgaggtg
       61 gccgagatgc caacacagat gtcaccaggg gcagtggaga tgtcaacacc tatgtcggca
      121 gagatgatgg agatgtcaac agaagtgact gagatgacac ctggggaggc ccttgcctca
      181 tccctcttct tccagcatca ccagttcatg tgctctgagt gtggcagcct ctataacaca
      241 ctggaggaag tcctctcaca ccaggagcag cacatgcttg ctgtctcaga ggaggaggca
      301 ctgaccacac agaatgttgg cctggagccg gagctggtgc cgggtgctga ggggcccttc
      361 cagtgtggtg aatgcagcca gctcatcctc tcccctgggg agctcctggc ccaccaggat
      421 gcccacctcc gagagtctgc aaaccagatc caataccagt gctgggactg ccaggagctg
      481 ttcccctcgc ccgagctgtg ggtggctcat cgaaaggccc agcacctttc tgctacggta
      541 gctgagccac cagtgccacc tcctttgcct cccccaacac cactgcctcc accttctccc
      601 ccatccgaag tcaagatgga gccctatgag tgtcctgagt gctctaccct ctgcgccacc
      661 cctgaggagt tcttggagca tcagggcacc cactttgact ccctagagaa agaggagcgc
      721 aatgggttgg aggaggagga agaggacgat gaggaggatg aagaagatga tgaagagatg
      781 gaggatgagg aggccatggc agaggtcggt gatgatgctg tgggaggtga cgagtccaca
      841 gctggctggg ctcagggctg cggggactgt ccccagcacc agccctcagc aggggctcgc
      901 cggcaacacc ggcggacggc tcacagcccg gcatctgcca cccacccctt ccactgcagc
      961 cagtgtcagc gcagtttcag ctccgccaac cggctgcagg ctcatgggcg ggcccatgtt
     1021 ggtggcacac atgagtgtac aacctgctcc aaggtcttca agaaagcagc atcgcttgag
     1081 cagcacttgc ggctgcatcg cggggaagcc cgctacctct gtgtagactg tggccgcggc
     1141 tttggcacag aactcacgtt ggtggctcac cggcgggccc acactgccaa cccattgcat
     1201 cgctgtcgtt gcggcaagac gttcagcaac atgaccaagt tcctctacca ccggcgcact
     1261 cacgccggca aaagcggggc acctcccaca ggagcaacag ctcccccagc tccagcggag
     1321 cccacccctc caccaccacc ccctgcccca cctgcccagc tgccctgccc acagtgctcc
     1381 aagtcctttg cctcagcttc ccggctgtcc cggcaccggc gtgcagtaca cgggccccct
     1441 gaacggcgtc accgctgtgg ggtttgtggc aagggcttca agaagctgat ccacgtgcgc
     1501 aaccacctgc ggacacacac gggtgagagg cccttccagt gccactcatg tggcaagacc
     1561 tttgcttctt tggccaacct cagccgccac cagctgaccc atacgggtgc acgtccctac
     1621 caatgcctgg actgtggcaa gcgcttcaca cagagctcca acctgcagca gcaccggcgg
     1681 ttgcacttgc ggccagtcgc ctttgcccgc gccccccgcc tccccatcac tggtctctac
     1741 aacaagagtc cctactactg cgggacttgt ggccgctggt tccgcgccat ggcgggcttg
     1801 cgactgcatc agcgggtcca tgcccgagct cggactttga cgctacagcc tcccagatca
     1861 ccatctcctg ccccaccccc acctccagag cctcaacaga ctatcatgtg cacagagctg
     1921 ggggagacca tcgccatcat tgagacatcc cagccactgg cgcttgagga caccctgcag
     1981 ctgtgccagg ctgcactggg ggccagtgaa gcaggcgggc tcttgcagtt ggacacggcc
     2041 ttcgtgtgcc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     2101 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     2161 tcttggcttt atatatcttg tggaaaggac gactgaatca agctcccacc ttgacc
//
