LOCUS       sequence-286158-        2225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3205
FEATURES             Location/Qualifiers
     source          1..2225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2057..2081)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2089..2130
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cagaggcacc ggcttccccg
       61 gccccgctct ctccgctcga agtggagctg gacccggagt tcgagcccca gagccgtccg
      121 cgatcctgta cgtggcccct gcaaaggccg gagctccaag cgagccctgc caagccctcg
      181 ggggagacgg ccgccgactc catgatcccc gaggaggagg acgatgaaga cgacgaggac
      241 ggcgggggac gggccggctc ggccatggcg atcggcggcg gcggcgggag cggcacgctg
      301 ggctccgggc tgctccttga ggactcggcc cgggtgctgg cacccggagg gcaagacccc
      361 gggtctgggc cagccaccgc ggcgggcggg ctgagcgggg gtacacaggc gctgctgcag
      421 cctcagcaac cgctgccacc gccgcagccg ggggcggctg ggggctccgg gcagccgagg
      481 aaatgttcgt cgcggcggaa cgcctgggga aacctgtcct acgcggacct gatcacccgc
      541 gccatcgaga gctccccgga caaacggctc actctgtccc agatctacga gtggatggtg
      601 cgttgcgtgc cctacttcaa ggataagggc gacagcaaca gctctgccgg ctggaagaac
      661 tccatccggc acaacctgtc actgcatagt cgattcatgc gggtccagaa tgagggaact
      721 ggcaagagct cttggtggat catcaaccct gatgggggga agagcggaaa agccccccgg
      781 cggcgggctg tctccatgga caatagcaac aagtatacca agagccgtgg ccgcgcagcc
      841 aagaagaagg cagccctgca gacagccccc gaatcagctg acgacagtcc ctcccagctc
      901 tccaagtggc ctggcagccc cacgtcacgc agcagtgatg agctggatgc gtggacggac
      961 ttccgttcac gcaccaattc taacgccagc acagtcagtg gccgcctgtc gcccatcatg
     1021 gcaagcacag agttggatga agtccaggac gatgatgcgc ctctctcgcc catgctctac
     1081 agcagctcag ccagcctgtc accttcagta agcaagccgt gcacggtgga actgccacgg
     1141 ctgactgata tggcaggcac catgaatctg aatgatgggc tgactgaaaa cctcatggac
     1201 gacctgctgg ataacatcac gctcccgcca tcccagccat cgcccactgg gggactcatg
     1261 cagcggagct ctagcttccc gtataccacc aagggctcgg gcctgggctc cccaaccagc
     1321 tcctttaaca gcacggtgtt cggaccttca tctctgaact ccctacgcca gtctcccatg
     1381 cagaccatcc aagagaacaa gccagctacc ttctcttcca tgtcacacta tggtaaccag
     1441 acactccagg acctgctcac ttcggactca cttagccaca gcgatgtcat gatgacacag
     1501 tcggacccct tgatgtctca ggccagcacc gctgtgtctg cccagaattc ccgccggaac
     1561 gtgatgcttc gcaatgatcc gatgatgtcc tttgctgccc agcctaacca gggaagtttg
     1621 gtcaatcaga acttgctcca ccaccagcac caaacccagg gcgctcttgg tggcagccgt
     1681 gccttgtcga attctgtcag caacatgggc ttgagtgagt ccagcagcct tgggtcagcc
     1741 aaacaccagc agcagtctcc tgtcagccag tctatgcaaa ccctctcgga ctctctctca
     1801 ggctcctcct tgtactcaac tagtgcaaac ctgcccgtca tgggccatga gaagttcccc
     1861 agcgacttgg acctggacat gttcaatggg agcttggaat gtgacatgga gtccattatc
     1921 cgtagtgaac tcatggatgc tgatgggttg gattttaact ttgattccct catctccaca
     1981 cagaatgttg ttggtttgaa cgtggggaac ttcactggtg ctaagcaggc ctcatctcag
     2041 agctgggtgc caggctgccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     2101 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     2161 atttcgattt cttggcttta tatatcttgt ggaaaggacg attaataagc ttctttgctc
     2221 caata
//
