LOCUS       sequence-286167-        1991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3208
FEATURES             Location/Qualifiers
     source          1..1991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1823..1847)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1855..1896
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cagctctgtc ccccacattt
       61 gccacgtcca cccaggattc cacctgtctc caggactcag aatttcctgt ttcttcaaaa
      121 gaccattcct gtccccagaa tttggacctg ttcgtgtgca gtggtctgga gcctcacaca
      181 cccagtgttg gttcccagga atcggtgact ttccaagatg tcgctgtgga ctttaccgag
      241 aaggagtggc ccttgctgga ttcttctcag agaaaactgt acaaagatgt aatgctggag
      301 aactacagca acctcacttc actggggtat caggtcggca aacccagcct catctcacac
      361 ttggagcagg aggaggagcc gaggacagag gagaggggcg ctcaccaggg cgcttgtgca
      421 gattgggaga ctccatctaa aaccaagtgg tcacttctta tggaagatat ttttgggaag
      481 gaaacgccca gtggtgtgac gatggaaaga gccggtcttg gagagaagtc cactgaatac
      541 gctcacttgt tcgaagtctt tggcatggac cctcatctca ctcagccaat gggaaggcac
      601 gctggcaaga ggccctatca ccgccgcgac tatggggtag cgttcaaggg caggccgcac
      661 ctcactcagc acatgagcat gtacgacggg agaaaaatgc atgaatgtca tcagtgccaa
      721 aaagccttca ccacgagcgc gtccctcaca cggcacagga gaatccacac cggggagaag
      781 ccttacgagt gcagcgactg cgggaaagcc ttcaacgacc cttcagccct taggagccac
      841 gcaagaactc acctcaaaga gaagcccttt gactgcagtc agtgtggaaa tgcattccgg
      901 accctctcgg ccctgaaaat ccacatgcga gttcacactg gcgagaggcc ttacaagtgt
      961 gatcagtgcg ggaaggctta cggccggagc tgccacctca tcgcacacaa gagaacgcac
     1021 accggagaga ggccctacga gtgtcacgac tgtgggaaag ctttccagca cccctcccac
     1081 ctcaaagagc acgtgaggaa tcacacgggg gagaagccct acgcgtgcac gcagtgcggc
     1141 aaagccttcc gctggaagtc caactttaat ttgcacaaga agaaccacat ggtggagaag
     1201 acctacgaat gtaaagaatg cgggaaatcc tttggcgatc tcgtgtcccg gaggaaacac
     1261 atgaggattc acatcgtcaa gaaacccgtg gaatgtcggc agtgcgggaa gaccttccga
     1321 aaccagtcca tccttaagac tcacatgaac tctcacactg gagagaaacc atacgggtgc
     1381 gatctctgcg ggaaagcttt cagcgcgagt tcaaacctca ccgcacacag gaagatacac
     1441 acgcaagaga gacgctacga atgcgccgcc tgcgggaaag tcttcggtga ctatttatcc
     1501 cggcggaggc acatgagcgt tcaccttgta aagaaacgag ttgagtgtag gcagtgtggc
     1561 aaggccttca ggaaccagtc aacgctgaag acgcacatgc gaagccacac gggggagaaa
     1621 ccgtacgaat gcgatcactg tgggaaggcc ttcagcatag gctccaacct gaatgtgcac
     1681 aggcggatcc acaccgggga gaagccctac gaatgccttg tctgcgggaa agccttcagc
     1741 gaccactcat ccctcaggag ccacgtgaaa actcaccggg gagagaagct ctttgtgtca
     1801 tccgtgtgga aaaggctcca gtgcccaact ttcttgtaca aagtggttga tatcggtaag
     1861 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1921 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaagt aaaaaacagc
     1981 ttataaacct c
//
