LOCUS       sequence-28617-e         443 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Rehwinkel J, Behm-Ansmant I, Gatfield D, Izaurralde E
  TITLE     A crucial role for GW182 and the DCP1:DCP2 decapping complex in 
            miRNA-mediated gene silencing.
  JOURNAL   RNA. 2005 Nov . 11(11):1640-7.
  PUBMED    16177138
REFERENCE   2  (bases 1 to 443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2005 
            Nov . 11(11):1640-7."
COMMENT     Sequence Label: pAc5.1A-Dm-mir-12
FEATURES             Location/Qualifiers
     source          1..443
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..26)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(36..77)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ggntactcat ggtgatggtg atgatgaccg gtacgcgtag aatcgagacc gaggagaggg
       61 ttagggatag gcttaccttc gaagggccct ctagactcga gctggccatt cgcctccttt
      121 aggctctcca ctgggcatga gaaaaggagc aaagattatg caggattagt aaccggaatt
      181 gaaagttgaa tgggcgttta aattagaggc atgatcatgt gagtggaact cgaagggagg
      241 cacatccgtt tgggtgaagc gcgcgcgttg gttgttggtc atgcggttag tgcgatccgt
      301 gcacggcgta gtatgacata agtactggtt gttggattta aggcacacca gtacctgatg
      361 taatactcaa ccgtacagac gctgctcctt cgtacagacg atccttcagt gcgtcgcggg
      421 caatgggttc ctattgccca atc
//
