LOCUS       sequence-286170-        1625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3209
FEATURES             Location/Qualifiers
     source          1..1625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1457..1481)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1489..1530
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg aaaccccaac acctttgccg
       61 cctgtacccg cctccccgac ctgcaaccca gccccacgga caatccagat cgagttccca
      121 cagcatagct cgtcgctgct ggaatctctg aaccgccaca ggctagaggg aaagttctgt
      181 gatgtgtccc tcctggtgca gggccgggaa cttagggctc ataaagcagt gttagctgct
      241 gcctctcctt acttccatga caagctgctt ctgggggatg cgcctcgtct cactctaccg
      301 agtgtcattg aagccgatgc cttcgagggg ctgctccagc tcatttattc agggcgtctc
      361 cgcctgccac tggatgctct tcctgctcat ctccttgtgg ccagtggcct tcaaatgtgg
      421 caggtagtag atcagtgctc agaaattctt agagaattag aaacttcagg tggtggaatt
      481 tcagcccgtg gaggaaactc ctaccatgcc cttctttcca ctacatcctc tacaggaggc
      541 tggtgcattc gctcttcgcc tttccagacc ccagtacagt cctctgcttc tactgaaagc
      601 cctgcttcca ctgagagccc tgtgggaggg gagggaagtg aactgggaga agtgctgcaa
      661 attcaggtgg aagaagaaga ggaggaggag gaagatgatg atgatgagga ccaggggtca
      721 gccacactct ctcagactcc tcagccccag agagtatcag gggtttttcc ccgtcctcat
      781 ggaccccacc cactgcccat gactgctact ccccgaaagc ttccagaggg tgagagtgca
      841 ccacttgagc ttcctgcccc tcctgcactg ccccccaaaa tcttctacat taagcaggaa
      901 cccttcgagc ctaaggagga gatatcagga agcggaactc agcctggagg agcaaaggag
      961 gaaaccaaag tgttttctgg aggggacact gaagggaatg gggagctagg gttcttgttg
     1021 ccttcagggc cagggccaac atctggggga gggggtccat cctggaaacc agtggatctt
     1081 catgggaatg aaatcctgtc agggggtgga ggacctgggg gagcaggcca ggccgtgcat
     1141 gggcctgtga agctaggggg gacaccccct gcagatggaa aacgctttgg ttgcctgtgt
     1201 gggaagcggt ttgcagtgaa gccaaagcgt gaccggcaca tcatgctgac cttcagcctt
     1261 cggccttttg gctgtggcat ctgcaacaag cgcttcaagc tgaagcacca tctgacagag
     1321 cacatgaaga cccatgctgg agccctgcat gcctgtcccc actgtggccg tcggttccga
     1381 gtccatgcct gttttctccg ccaccgggac ctatgcaagg gccagggctg ggccactgcc
     1441 cactggactt acaagtgccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     1501 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1561 atttcgattt cttggcttta tatatcttgt ggaaaggacg aatttaattt gtcagttgaa
     1621 cttca
//
