LOCUS       sequence-286173-        1292 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3210
FEATURES             Location/Qualifiers
     source          1..1292
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1124..1148)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1156..1197
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgacggggaa agcaggggaa
       61 gcgctgagca agcccaaatc cgagacagtg gccaagagta cctcgggggg cgccccggcc
      121 aggtgcactg ggttcggcat ccaggagatc ctgggcttga acaaggagcc cccgagctcc
      181 cacccgcggg cagcgctcga cggcctggcc cccgggcact tgctggcggc gcgctcagtg
      241 ctcagccccg cgggggtggg cggcatgggg cttctggggc ccggggggct ccctggcttc
      301 tacacgcagc ccaccttcct ggaagtgctg tccgacccgc agagcgtcca cttgcagcca
      361 ttgggcagag catcggggcc gctggacacc agccagacgg ccagctcgga ttctgaagat
      421 gtttcctcca gcgatcgaaa aatgtccaaa tctgctttaa accagaccaa gaaacggaag
      481 aagcggcgac acaggacaat ctttacctct taccagctag aggagctgga gaaggcattc
      541 aacgaagccc actacccaga cgtctatgcc cgggagatgc tggccatgaa aacggagctg
      601 ccggaagaca ggatacaggt ctggttccag aaccgtcgag ccaagtggag gaagcgggag
      661 aagtgctggg gccggagcag tgtcatggcg gagtatgggc tctacggggc catggtgcgg
      721 cactccatcc ccctgcccga gtccatcctc aagtcagcca aggatggcat catggactcc
      781 tgtgccccgt ggctactggg gatgcacaaa aagtcgctgg aggcagcagc cgagtcgggg
      841 aggaagcccg agggggaacg ccaggccctg cccaagctcg acaagatgga gcaggacgag
      901 cggggccccg acgctcaggc ggccatctcc caggaggaac tgagggagaa cagcattgcg
      961 gtgctccggg ccaaagctca ggagcacagc accaaagtgc tggggactgt gtctgggccg
     1021 gacagcctgg cccggagtac cgagaagcca gaggaggagg aggccatgga tgaagacagg
     1081 ccggcggaga ggctcagtcc accgcagctg gaggacatgg ctttgccaac tttcttgtac
     1141 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     1201 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1261 aaggacgata gtcttagcac ctttacgata at
//
