LOCUS       sequence-286182-        1796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3213
FEATURES             Location/Qualifiers
     source          1..1796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1628..1652)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1660..1701
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggagcaaga aaaaaaactg
       61 ttggtctcag attctaacag ctttatggag agggagagtt tgaaaagccc tttcacagga
      121 gatacaagta tgaataattt ggaaactgtt caccacaata attctaaggc agataaactt
      181 aaagagaaac cttcagaatg gtctaaaaga catagaccac aacattataa gcatgaggat
      241 gcaaaagaaa tgccactgac atgggttcaa gatgagattt ggtgtcatga ttcctatgag
      301 agtgatggca agtcagagaa ttggggaaat tttatagcta aagaggagga aaaacccaat
      361 caccaggaat gggactcagg agaacatacc aatgcctgtg tccagcagaa ttcatccttt
      421 gtagacagac cctataaatg ttccgaatgt tggaaaagct tcagtaatag ttctcatttg
      481 cgtactcacc agaggaccca ctcaggagaa aagccttata aatgctctga gtgtgcaaaa
      541 tgtttttgta acagttctca cctgattcag catctaagaa tgcacacagg agagaagccc
      601 taccagtgtg gtgaatgtgg gaaaagcttc agcaatacct cccatcttat tatccatgag
      661 agaactcaca cgggagagaa accctacaaa tgtcccgagt gtgggaagag attcagcagc
      721 agctctcacc ttattcagca tcacagatca catacaggtg aaaaaccata tgaatgttct
      781 gtctgcggaa aaggcttcag tcacagctat gtcctaatag aacatcagag gactcacact
      841 ggagaaaaac cttataagtg ccctgattgt gggaagagtt ttagtcagag ttccagcctc
      901 attcgccacc agcggacaca cacaggtgag aagccctaca aatgtcttga gtgtgaaaaa
      961 agctttggtt gtaattctac tctaataaaa catcagagaa tacatacagg agaaaagcct
     1021 tatcaatgtc cagaatgtgg gaagaatttt agtcgtagtt caaaccttat tacacaccag
     1081 aaaatgcaca caggagagaa atcctatgaa agttctgaat atgaggaaag tttgggtcag
     1141 aactgcaatg tgatagaaga atgcagaatc cagttaggag agaaaccata tagatgttgt
     1201 gaatgtggga agagttttgg ccttagctcc catctcatta gacatcagag aacacataca
     1261 ggagaaaaac cttacagatg ttctgagtgc tggaaaactt tcagtcagag ttccaccctg
     1321 gtgattcacc aaaggacaca tacaggagag aaaccttata aatgtcctga ttgtggtgaa
     1381 agcttcagtc agagctttaa ccttatcagg caccggagga cccacatagg ggaaaaacct
     1441 tacaaatgta ccagctgtga gaaatgcttc agcagaagtg cctacctcag tcagcatcgg
     1501 aaaattcacg tagaaaagcc ttttgagtct cccgacgttg gggattttcc tcatgaatgg
     1561 acttggaaaa actgttcagg ggaaatgccc ttcatctctt cattttccgt ctcaaattca
     1621 tcttccttgc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1681 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1741 tcttggcttt atatatcttg tggaaaggac gaaactatgc aattcacttt atttgc
//
