LOCUS       sequence-286185-        2765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3214
FEATURES             Location/Qualifiers
     source          1..2765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2597..2621)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2629..2670
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgaccaagtc cctggaatca
       61 gtgtcattca aggacgtaac tgtagacttc agtagggatg agtggcaaca attagacctt
      121 gctcagaaaa gcctgtacag agaagtgatg ctggaaaact atttcaactt gatctcagtg
      181 ggatgtcaag ttcccaaacc agaagtcatc ttcagcttgg aacaagaaga gccatgtatg
      241 ttggatggtg agatccccag tcagagccgt ccagatgggg acattggttt tggaccttta
      301 caacagagga tgtctgaaga agtttctttc cagtctgaga ttaatattaa tctcttcaca
      361 agagatgacc catattccat tttagaagaa ttgtggaaag acgatgaaca cacaagaaaa
      421 tgtggagaaa accagaacaa acctttaagt cgtgttgtct tcattaacaa gaaaacacta
      481 gctaatgaca gcatctttga atataaggac attggggaaa tagttcatgt aaacacacac
      541 ctggtttcct caagaaaaag accccataac tgtaactcgt gtggaaagaa tttggagcct
      601 atcataacct tatataatag aaacaatgca acagaaaatt ctgataagac tattggagat
      661 ggtgatattt tcactcattt gaattctcat acagaagtga ctgcttgtga atgtaaccaa
      721 tgtgggaaac ctctgcatca taagcaagct ctcattcaac aacagaaaat tcatactaga
      781 gagagcctct atttgttttc tgactacgta aatgttttct ccccgaagtc acatgccttt
      841 gcacatgaga gtatttgtgc tgaagaaaag cagcatgaat gccatgaatg tgaggcagtc
      901 ttcactcaga agtcccagct tgatggcagt cagagggttt atgcaggaat atgcactgaa
      961 tatgagaagg atttttccct caagtcaaac cgtcagaaaa ctccttatga ggggaattac
     1021 tataaatgca gtgactatgg aagagccttt atccagaagt cagatctgtt cagatgccag
     1081 agaattcatt ctggagaaaa accttatgag tacagtgaat gtgagaaaaa cctccctcag
     1141 aattcaaacc ttaatataca taaaaaaatt catactggag ggaaacactt tgaatgtact
     1201 gaatgtggaa aagctttcac aaggaaatca acactaagta tgcatcagaa aatccataca
     1261 ggagaaaaac cttatgtatg tactgaatgt gggaaggcct ttatccggaa gtcacatttt
     1321 atcacacatg aaagaattca tactggagaa aaaccctatg aatgcagtga ctgtgggaaa
     1381 tcctttatta aaaaatcaca actccatgtg catcagcgaa ttcacacagg agagaatccc
     1441 tttatatgtt cagaatgtgg gaaggtcttc actcacaaga caaatctcat tatacaccag
     1501 aaaattcata caggagaaag accctatata tgtactgtgt gtggtaaggc ctttactgac
     1561 aggtcaaatc tcattaagca ccaaaaaatt catactggag agaaacctta taaatgcagc
     1621 gactgtggaa aatcattcac ctggaagtct cggctcagga tacatcagaa gtgtcatact
     1681 ggagagagac attatgaatg cagtgaatgt gggaaagcat tcattcagaa gtcaacatta
     1741 agtatgcacc agagaattca tagaggggaa aaaccatatg tttgcactga atgtggtaag
     1801 gccttcttcc acaaatccca ttttattaca catgagagaa ttcatactgg agagaaaccc
     1861 tatgaatgca gtatttgtgg gaaatccttc actaagaaat cacagctcca cgtacatcag
     1921 cagattcaca caggagagaa accctatagg tgtgctgaat gtggaaaggc ttttactgac
     1981 agatcaaatc tctttacaca ccagaaaatt cacactggag agaaacctta taaatgtagt
     2041 gactgtggga aagccttcac taggaagtca ggtctccata tacatcagca atcccatact
     2101 ggagaaaggc attatgagtg cagtgaatgt gggaaagcct ttgcaagaaa atcaacacta
     2161 attatgcatc agagaattca tacaggagag aaaccctata tttgtaatga atgtgggaaa
     2221 tccttcatcc agaagtcaca cttaaataga cacaggagaa ttcatactgg agagaaaccc
     2281 tatgaatgca gtgactgtgg caagtctttc attaagaaat cacaactcca tgagcatcat
     2341 cgaattcaca caggagagaa accatatata tgtgctgagt gtggaaaggc cttcaccatc
     2401 agatcaaatc ttattaaaca ccagaaaatt catactaaac agaaacccta taagtgcagt
     2461 gacttgggga aagccttaaa ctggaagcca caactcagta tgcctcagaa atctgacaat
     2521 ggggaagtag agtgctccat gccacaatta tggtgtgggg actcagaagg tgaccaaggc
     2581 caactttctt ctatcttgcc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     2641 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     2701 atttcgattt cttggcttta tatatcttgt ggaaaggacg agtgcagatc ggtctctatc
     2761 ggctt
//
