LOCUS       sequence-286188-        1946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3215
FEATURES             Location/Qualifiers
     source          1..1946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1778..1802)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1810..1851
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgaacaaatc ccaggaacaa
       61 gtgtcattca aggatgtatg tgtggacttc actcaggaag agtggtatct gctggaccct
      121 gctcagaaga ttctatacag agatgtgatc ctggaaaatt atagcaatct tgtctcagta
      181 gggtattgca ttactaaacc agaagtgatc tttaagatcg agcaaggaga agagccctgg
      241 atattagaaa aaggattccc aagccagtgc cacccagaaa ggaaatggaa agttgatgac
      301 gtgttagaga gcagccagga aaatgaagat gaccattttt gggagcttct attccacaac
      361 aacaaaacag taagtgtaga aaatggagat agaggaagca aaactttcaa tttgggcaca
      421 gaccctgttt ctttaagaaa ttatccctat aaaatatgtg actcatgtga aatgaatttg
      481 aaaaatattt cgggcttaat tattagtaaa aagaactgtt ccagaaagaa gcctgatgag
      541 tttaatgtat gtgagaaatt gctccttgat attaggcatg agaaaatccc tattggagag
      601 aagtcttata aatatgatca aaaaaggaat gccattaatt atcaccagga tctcagtcag
      661 ccaagttttg gccaatcttt tgagtatagt aaaaatggac aaggcttcca tgatgaggca
      721 gcatttttta caaataagag atctcagata ggagagacag tctgtaaata taacgaatgt
      781 ggaagaacct tcattgaaag tttaaagctg aatatatctc aaagacctca tttggaaatg
      841 gagccgtatg gatgcagtat ttgcgggaag tccttctgca tgaatttaag gtttggacat
      901 cagagagctc ttacaaagga caatccttat gaatataatg aatatgggga aatcttctgt
      961 gacaattcag ctttcattat ccatcaggga gcttacacaa gaaagattct ccgtgaatat
     1021 aaagtgagtg acaaaacctg ggaaaagtca gctctcttaa aacatcaaat agtacacatg
     1081 gggggaaagt cttatgatta caatgaaaat gggagtaatt tcagcaagaa gtcacatctt
     1141 acccagcttc ggagagctca cacaggagaa aaaacctttg aatgtggtga atgtgggaaa
     1201 accttctggg agaagtcaaa cctcactcaa catcagagaa cacacacagg agagaagccc
     1261 tatgaatgta ctgaatgtgg gaaagccttt tgccagaaac cacacctgac caaccatcag
     1321 cgaacacata caggagaaaa accctatgaa tgtaagcaat gtggaaaaac attctgtgtg
     1381 aagtcaaacc tcactgaaca tcagagaaca cacacagggg agaagcccta tgaatgtaat
     1441 gcatgtggga aatccttctg ccacagatca gccctcactg tgcatcagag aacacacaca
     1501 ggggagaaac cgtttatatg taatgaatgt ggaaaatcct tctgtgtgaa gtcaaacctc
     1561 attgtacatc aaagaactca cactggggag aaaccatata agtgtaatga atgtgggaaa
     1621 accttctgtg aaaaatcagc tctcactaaa catcagagga ctcacacagg ggagaagccg
     1681 tatgagtgta atgcatgtgg gaagaccttt agtcagaggt cagtgctcac caaacatcag
     1741 agaattcaca caagggtgaa agctctttca acatccttgc caactttctt gtacaaagtg
     1801 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     1861 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1921 gacgtatgcc gattttcacc gccaac
//
