LOCUS       sequence-286191-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3216
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(665..689)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             697..738
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga aattcaagtt acatgtgaat
       61 tctgccaggc aatacaagga cctgtggaat atgagtgatg acaaaccctt tctatgtact
      121 gcgcctggat gtggccagcg ttttaccaac gaggatcatt tggctgtcca taaacataaa
      181 catgagatga cactgaaatt tggtccagca cgtaatgaca gtgtcattgt ggctgatcag
      241 accccaacac caacaagatt cttgaaaaac tgtgaagaag tgggtttgtt taatgagttg
      301 gcgagtccat ttgagaatga attcaagaaa gcttcagaag atgacattaa aaaaatgcct
      361 ctagatttat cccctcttgc aacacctatc ataagaagca aaattgagga gccttctgtt
      421 gtagaaacaa ctcaccagga tagtccttta cctcacccag agtctactac cagtgatgag
      481 aaggaagtac cattggcaca aactgcacag cccacatcag ctattgttcg tccagcatca
      541 ttacaggttc ccaatgtgct gcttacaagt tctgactcaa gtgtaattat tcagcaggca
      601 gtaccttcac caacctcaag tactgtaatc acccaggcac catcctctaa caggccaatt
      661 gtgtacccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
      721 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
      781 tggctttata tatcttgtgg aaaggacgac ccggtggtga ccgctccggt cga
//
