LOCUS       sequence-286197-        1610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3218
FEATURES             Location/Qualifiers
     source          1..1610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1442..1466)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1474..1515
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg gtccagtcat gcctcccagt
       61 aagaagccag aaagctcagg aattagtgtc tccagtggac tgagtcagtg ttacgggggc
      121 agcggtttct ccaaggccct tcaggaagac gatgacctcg acttttctct gcctgacatc
      181 cgattagaag agggggccat ggaagatgaa gagctgacca acctgaactg gctgcacgag
      241 agcaagaact tgctgaagag ctttggggag tcggtcctca ggagtgtcag ccccgtccag
      301 gacctggacg atgacacccc cccatcccct gcccactctg acatgcccta cgatgccagg
      361 cagaacccca actgcaaacc cccctactcc ttcagctgcc tcatatttat ggccatcgag
      421 gactctccaa ccaagcgcct gccagtgaag gatatctaca actggatctt ggaacatttt
      481 ccgtattttg caaatgcacc tactgggtgg aaaaactcag tgagacacaa tttatcattg
      541 aataagtgtt ttaagaaagt ggacaaagag aggagtcaga gtattgggaa agggtcgttg
      601 tggtgcatag acccagagta tagacaaaat ctaattcagg ctttgaaaaa gacaccttat
      661 cacccacacc cacacgtgtt caatacacct cccacctgtc ctcaggcata tcaaagcaca
      721 tcaggtccac ccatctggcc gggcagtacc ttcttcaaga gaaatggagc ccttctccaa
      781 gttcctccag gagtgatcca aaatggagcg cgggtcctga gccgagggct gtttcctggc
      841 gtgcggccgc tgccaatcac tcccattggg gtgacagcgg ccatgaggaa tggcatcacc
      901 agctgccgga tgcggactga gagtgagcca tcttgtggct ccccagtggt cagcggagac
      961 cccaaggagg atcacaacta cagcagtgcc aagtcctcca acgcccggag cacctcgccc
     1021 accagcgact ccatctcctc ctcctcctcc tcagccgacg accactatga gtttgccacc
     1081 aaggggagcc aggagggcag cgagggcagc gaggggagct tccggagcca cgagagcccc
     1141 agcgacacgg aagaggacga caggaagcac agccagaagg agcccaagga ttctctgggg
     1201 gacagcgggt acgcatccca gcacaagaag cgccagcact tcgccaaggc caggaaggtc
     1261 cccagcgaca cactgcccct caaaaagaga cgcaccgaaa agccccccga gagcgatgat
     1321 gaggagatga aagaagcggc agggtccctc ctgcacttag cagggatccg gtcctgtttg
     1381 aataacatca ccaatcggac ggcaaagggg cagaaagagc aaaaggaaac cacaaaaaat
     1441 tacccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     1501 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1561 ctttatatat cttgtggaaa ggacgaaggg atcatcggat ttccatgcct
//
