LOCUS       sequence-286200-        1958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3219
FEATURES             Location/Qualifiers
     source          1..1958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1790..1814)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1822..1863
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ctctttctca gggactgttt
       61 acattcaagg atgtggccat agaattctct caagaggagt gggagtgcct ggaccctgcc
      121 cagagggcct tgtacaggga cgtgatgttg gagaactaca ggaacctgct ttctctcgat
      181 gaggataaca tccctccaga agatgatatt tctgttggat ttacaagcaa gggattatca
      241 ccaaaggaaa ataataaaga ggaattatac catctggtga tattagaaag aaaggaaagc
      301 catggcatca acaattttga cctcaaggaa gtctgggaaa atatgcctaa gtttgacagc
      361 ctgtgggact atgatgtaaa aaattacaaa ggaatgcctt tgacctgtaa caaaaatctc
      421 actcacagaa aagatcaaca acataataaa tcctcaatac atttctcttt aaagcagagt
      481 gtttctataa gagatagtgc acaccagtat ttcatccatg acaagccatt tataaggaat
      541 ttgttaaaac tgaaaaataa cataaggtat gccggaaaca aatacgtgaa gtgttttgaa
      601 aataaaattg gattaagctt acaggcacag ctggctgaac tacagagatt tcaaactggg
      661 gagaaaatgt atgaatgtaa tccagttgag aagtctatca atagttcctc agtttcacca
      721 cttcctcctt gtgtcaaaaa catttgtaat aaatatagga agattttgaa atacccttta
      781 ttacatacac agtatgggag aacacacatt agagaaaaat catacaagtg taatgactgt
      841 ggaaaggctt ttagcaaaag ttcgaacctc actaatcatc agagaattca ctctggacag
      901 agaccttaca aatgtaacga gtgtggcaaa gcctttaacc agtgttcgaa cctcactagg
      961 catcagagag tccatacagg agagaaacca tatcaatgta atatatgtgg caaggtctgt
     1021 agtcaaaatt caaatcttgc aagtcatcag aggatgcata ctggagagaa accttacaaa
     1081 tgtaatgaat gtggtaaggc atttatccag cgttcacacc tttggggtca tgaaagaatt
     1141 catactggag agaaacctta caaatgtaat gaatgtgaca aagcctttgc tgaacgttca
     1201 agccttaccc aacataagag aatccatact ggagagaagc cttacatatg taatgagtgt
     1261 ggcaaagctt ttaagcagtg ctcacatctc actaggcatc agaatataca tcctggagag
     1321 aaaccacaca aatgtaatgt gtgtggcagg gcttttatcc aaagttcaag tcttgtggaa
     1381 catcagagaa ttcacactgg agaaaaacct tacaaatgta ataaatgtga taaagctttt
     1441 atcaaacgtt cacacctttg gggtcatcag agaactcata ctggagagaa accttacaaa
     1501 tgtactgaat gtggcaaagc ctttactgaa cgttcaaatc ttactcagca taagaaaatc
     1561 catactggag agaaacctta caaatgtact gaatgtggca aagcttttac ccaatttgca
     1621 aacctcacta gacaccagaa aatacacatt gaaaagaaac attgtaaaca taatatacat
     1681 ggtaatgctt tattccaaag ttcaaacctt ggagatcatc aaaaaagtta taatagagaa
     1741 aaacatatca aatataatga gacaaaaatt aagtattcaa gctgtaccta cccaactttc
     1801 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
     1861 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1921 tgtggaaagg acgaggcata cggctcagga ggtccggt
//
