LOCUS       sequence-286203-        1760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3220
FEATURES             Location/Qualifiers
     source          1..1760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1592..1616)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1624..1665
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ctgtggccct gggttgtgca
       61 atccaggcat ccttgaatca aggctctgtg tttcaagaat atgatactga ctgtgaagtt
      121 ttccgtcagc gcttcaggca gttccagtac agagaagcag ctgggcctca tgaagcattt
      181 aacaaactct gggagctttg ctgtcaatgg ctgaagccaa agatgcgctc taaggaacaa
      241 atcctggagc tgctagtgtt ggagcaattc ctaactatcc tgcccacaga gatagagacc
      301 tgggtgaggg agcactgccc agagaataga gaaagagttg tgtcactgat agaagactta
      361 cagagagaac ttgagatacc agagcagcag gttgatatgc atgacatgct cttggaagaa
      421 ctggcaccag tgggaacggc acacatacca ccaaccatgc acctagagtc acctgcactc
      481 caggtaatgg gacctgccca ggaggcccca gtagcagagg catggatccc acaggcaggg
      541 ccaccggagc tgaactatgg tgctactgga gaatgtcaga actttctgga ccctggatat
      601 ccattaccaa aacttgacat gaacttctca ttggagaata gagaagagcc atgggtgaag
      661 gaattacagg attctaaaga aatgaaacaa ttacttgatt ccaagatagg ttttgagatc
      721 gggatagaaa atgaagaaga tacttcaaaa cagaaaaaaa tggagactat gtatccattt
      781 attgtaactt tagaggggaa tgctctccag ggtcccattt tgcaaaaaga ctatgtacag
      841 ttagaaaatc aatgggaaac ccccccagag gatttacaga cagatttagc aaaactggta
      901 gatcagcaga accccactct gggagagaca cctgagaact ccaacttgga agaacctctc
      961 aaccctaaac cccacaagaa aaagagtcca ggagagaaac ctcaccgatg tcctcagtgt
     1021 ggaaaatgtt ttgctcggaa gtcacaactt actgggcatc agagaattca ttcaggagaa
     1081 gaacctcaca aatgccctga atgtgggaaa agattccttc gtagttcaga cctttataga
     1141 caccaacgac ttcatacagg ggagagaccc tatgaatgca ctgtatgtaa aaagcgattc
     1201 actcggcggt cacatcttat agggcaccag agaacccatt ctgaagaaga aacatataaa
     1261 tgtcttgagt gtgggaaaag tttttgtcat ggatcaagtc ttaaaagaca tctgaaaact
     1321 catacaggtg aaaaacctca tagatgtcat aattgtggga aaagttttag tcgactgaca
     1381 gctcttactt tgcaccagag aacgcatact gaagagagac cttttaaatg taattattgt
     1441 gggaaaagtt ttagacagag accaagcctc gttattcatt taagaatcca cacaggggag
     1501 aagccataca agtgtactca ttgttctaaa agcttcagac agagagccgg ccttattatg
     1561 caccaggtca ctcactttag aggacttatt tacccaactt tcttgtacaa agtggttgat
     1621 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     1681 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgagtaa
     1741 ctgggttacg accgtatgtt
//
