LOCUS       sequence-286206-        2279 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3221
FEATURES             Location/Qualifiers
     source          1..2279
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2111..2135)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2143..2184
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga tggctgaaaa caatttaaaa
       61 atgctaaaga ttcaacagtg tgtggtagcc aacaaactac ctagaaacag gccatatgtt
      121 tgcaatattt gttttaagca ctttgaaaca ccatcaaaat tagctaggca ctatctcatt
      181 catactggtc aaaagccatt tgaatgtgat gtgtgtcata aaacctttag acaactagtt
      241 catctggaga ggcatcaact aactcatagt ctgcctttta aatgtagtat ttgtcagcgt
      301 cactttaaaa atctgaagac atttgtgaag caccaacaac ttcacaatga aacctatcag
      361 aataatgtta aacaggtcag aagattgctg gaggccaagc aagaaaagtc aatgtatgga
      421 gtgtataata cttttaccac agaggaaaga tgggcattac acccgtgctc taagtctgat
      481 cccatgtata gcatgaaaag aagaaagaat attcatgcat gtacaatctg tggcaagatg
      541 tttccatcac agtcaaaact tgataggcat gtacttattc atactggtca gaggcctttt
      601 aaatgtgtct tgtgtactaa atcttttcga cagtcaactc acttaaaaat ccaccaactt
      661 acacattcag aagaaagacc ttttcaatgt tgtttttgtc aaaaaggatt taagattcaa
      721 agcaaacttc tgaagcataa acaaatccat actaggaata aggcttttcg ggctctttta
      781 ttaaagaaga ggcgtacaga atctcgcccc ctgcctaata agttaaatgc aaatcagggt
      841 ggttttgaaa atggtgagat tggtgaatct gaggagaata atccacttga tgtccactca
      901 atttatattg tcccttttca atgtccaaag tgtgaaaagt gttttgaatc agagcagatt
      961 ctcaatgaac acagctgttt tgctgctaga agtggcaaaa ttccaagcag gttcaaaaga
     1021 agctacaact ataaaaccat tgttaaaaaa atcttggcca agcttaagcg tgctaggagt
     1081 aaaaaattag ataactttca atctgagaaa aaagttttta aaaagagttt cttgagaaat
     1141 tgtgatctta tttctggtga gcagagctct gaacaaaccc agagaacatt tgtgggttct
     1201 cttggcaaac atggaacata taaaacaatt ggcaatagaa agaagaaaac attgactttg
     1261 ccattttctt ggcaaaatat gggaaaaaat ttgaaaggca tccttacgac agaaaacata
     1321 ttaagcattg ataattcagt gaataagaaa gacttgtcaa tctgtggttc atcaggtgag
     1381 gaattcttta ataactgtga ggtacttcag tgtggttttt cagttccaag ggaaaacata
     1441 cgtactagac ataagatatg tccttgtgac aaatgtgaga aggtatttcc ttctatatcc
     1501 aaactaaaaa gacactattt aattcatact ggacagaggc cctttggctg taatatttgt
     1561 gggaaatctt ttagacagtc agctcactta aaaagacatg aacagactca taatgaaaag
     1621 agtccttatg catctctttg ccaagtagaa tttggaaact tcaacaatct ttctaatcat
     1681 tcaggtaata atgttaacta taatgcttcc caacaatgtc aggctcctgg tgttcaaaaa
     1741 tacgaggtct cagagtcaga tcaaatgtca ggagttaagg cagagtcaca ggattttatt
     1801 cctggtagca ccgggcaacc ctgtcttcct aatgtacttt tggaatcaga gcaaagcaat
     1861 cctttttgca gttattcaga gcatcaggag aaaaatgatg tcttcctgta ccgatgcagt
     1921 gtttgtgcta aaagtttccg atctccatct aaactggaaa gacactacct aattcatgca
     1981 gggcagaaac catttgaatg ctcagtttgt ggcaaaacat tcagacaggc tcctcactgg
     2041 aagagacatc agcttactca ctttaaagaa cgaccacaag ggaaagtggt tgccttagat
     2101 tcggttatgt acccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     2161 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     2221 atttcttggc tttatatatc ttgtggaaag gacgaaaaac ccagttatgg cctggagac
//
