LOCUS       sequence-286209-        1196 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3222
FEATURES             Location/Qualifiers
     source          1..1196
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1028..1052)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1060..1101
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc agcagcagcc tctgcccggg
       61 cctggcgccc ccacaactga gccaaccaag cctccctaca gctacatcgc ccttattgct
      121 atggccatcc agagctcacc ggggcagcgg gccaccctca gtggcatcta ccgctacatc
      181 atgggccgat tcgccttcta ccgccacaac cggcccggct ggcagaacag catccgccac
      241 aacctgtcac tcaacgagtg ctttgtcaag gtgccccgcg atgaccgcaa gccaggcaag
      301 ggcagctact ggacgctgga ccctgactgc cacgacatgt ttgagcacgg cagcttccta
      361 cgccgccgcc gccgcttcac ccggcagaca ggtgctgagg gcacccgggg ccccgccaag
      421 gcacgccgtg gacccctcag ggcgaccagc caggacccag gagtccccaa cgccacgacc
      481 ggcaggcagt gctcattccc accagagctg ccagatccca agggcctaag ctttgggggt
      541 ctggtggggg ccatgccagc cagtatgtgc ccagcaacca ctgatggcag gcctcggcca
      601 cccatggagc ccaaagagat ttccacgccc aagcctgcat gcccagggga gctccccgtg
      661 gccacctcat cttcctcatg cccagcgttt ggctttcctg ccggcttctc agaggctgag
      721 agttttaata aggcccctac gcccgtcttg tccccggaat caggcatcgg gagcagctac
      781 cagtgtcggc tgcaggcact gaatttttgc atgggggctg acccaggcct tgagcacctc
      841 ttggcctcag cagccccctc ccctgcacca cccacccctc caggctcact ccgggcccca
      901 ctgcccctgc caactgacca caaggaaccc tgggttgcag gtggcttccc tgtccaggga
      961 ggctccggct acccattggg gctgaccccc tgcctatacc ggacgccagg catgttcttc
     1021 tttgagtacc cgactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1081 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1141 tcttggcttt atatatcttg tggaaaggac gacgaaggag gaagaataat tgacta
//
