LOCUS       sequence-286212-        2795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3223
FEATURES             Location/Qualifiers
     source          1..2795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2627..2651)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2659..2700
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgaactccat gaaccccatg
       61 aaacctgccc tgccccctgc gccacacggt gatggttcat tcgcatatga gtctgtgcct
      121 tggcaacaaa gcgccactca gccggctgga tcgctgtctg tggtcactac tgtgtgggga
      181 gttggcaacg cgacacagag ccagtgcctg ggacagcagg cgtttgctga aggcggcgcc
      241 aacaagggct acgtgcagca aggcgtgtac agccgcgggg gctaccctgg ggcccccggc
      301 ttcaccaccg ggtatgcagg cggcccgggg ggcctgggcc tcccctcaca tgctgcaaga
      361 ccctccactg acttcacgca agcggcagct gctgcagctg tggctgctgc ggcagccact
      421 gccaccgcca cagccacagc caccgtggct gctctccagg agaagcagag ccaggagctg
      481 agccagtatg gagcgatggg ggccggacag tcttttaaca gccagtttct gcagcatgga
      541 ggtccccggg ggcctagtgt ccccgctggc atgaacccta ctggcatagg aggggtaatg
      601 ggcccctctg gcctctcccc cttggctatg aaccccaccc gggcagcagg aatgacaccc
      661 ttgtatgcag ggcagcgtct gccccaacat gggtatcctg ggcctcccca ggcccagcca
      721 ctgccccgac agggggtcaa gagaacctac tctgaggtgt atccagggca gcagtatctg
      781 caaggaggcc agtatgcacc cagcaccgcc cagtttgcgc ccagccctgg gcagccccct
      841 gccccctccc cttcctaccc tgggcacagg ctgcccctgc agcagggcat gacccagtcc
      901 ctgtccgtgc ctggccccac gggactgcat tataagccta cccgttccat cccgggctat
      961 cccagttccc cactgccagg gaaccccacg ccacccatga ccccaagcag cagcgtccct
     1021 tacatgtcac caaaccaaga ggtcaagtct cccttcttgc ctgatctcaa gcccaacctc
     1081 aactccttgc actcatcgcc ctctggaagc gggccttgtg acgagttgcg gctgaccttc
     1141 cctgtgcgcg atggggtggt cctggagccc ttccgcctgc agcacaacct ggctgtaagc
     1201 aaccatgtct tccagctgcg agactcagtc tacaagaccc tgataatgag gcctgacctg
     1261 gagctgcaat tcaagtgcta ccaccacgag gaccggcaga tgaacaccaa ctggccagcc
     1321 tcggtgcagg tcagcgtcaa tgccacgccg ctcaccatcg agcgtggcga caacaagacc
     1381 tcgcacaagc cactctacct gaagcatgtg tgccagccag gccgcaacac catccagatc
     1441 accgtcaccg cctgctgctg ctcccacctc ttcgtgctgc agctagtgca ccgcccatcc
     1501 gtccgctcgg tgctgcaggg cctcctcaaa aagcgcctcc tgcctgctga gcactgcatc
     1561 accaagataa agcggaactt cagcagcggc accatccctg gcacccctgg gcccaacgga
     1621 gaggacgggg tggagcagac agctatcaag gtgtccctga agtgccccat caccttccgc
     1681 aggatccagc tccctgcccg aggtcatgac tgtcgccaca tacagtgctt tgacctggag
     1741 tcgtacctgc agctcaactg tgagcggggg acttggaggt gtcctgtgtg caacaagaca
     1801 gctttgctgg agggcctgga ggtggaccag tacatgctgg gcatcctgat ttacattcag
     1861 aactctgact atgaggagat caccatcgac cccacgtgca gctggaagcc agtgcccgtg
     1921 aagcctgaca tgcacatcaa ggaggagccg gatgggccag cactgaagcg ctgccgcacc
     1981 gtgagccccg cccacgtgct catgcccagc gtgatggaga tgatcgccgc cctgggcccc
     2041 ggcgctgccc cctttgcccc cctgcagccc ccctcagtcc ctgcccccag cgactaccct
     2101 ggccagggtt ccagcttcct ggggcctgga actttccctg agtccttccc acccaccacg
     2161 cccagcaccc caacccttgc tgagttcacc ccgggaccac cccccatctc ctaccagtct
     2221 gacattccca gcagcctcct gacttcagag aagtctaccg cctgcctccc aagccagatg
     2281 gcaccagcag gtcacctgga ccccactcac aatcctggga caccaggact acacacctcc
     2341 aaccttgggg cccctccagg tccccagctg caccattcaa accctccccc agcgtcccgg
     2401 cagtccttgg gccaagcgag cttaggacct acgggtgaac tggccttcag tcctgccaca
     2461 ggcgtgatgg ggccccccag catgtctgga gccggggagg ccccagaacc agctctggac
     2521 ctgctcccgg aactgaccaa ccctgatgag ctactgtcct acttgggccc acccgacctc
     2581 cctacgaaca acaatgacga cctgctttct ctgtttgaga acaacttgcc aactttcttg
     2641 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     2701 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2761 ggaaaggacg aattattttc cttccacatt tacat
//
