LOCUS       sequence-286218-        1979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3225
FEATURES             Location/Qualifiers
     source          1..1979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1811..1835)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1843..1884
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgattaagtt ccaggaaagg
       61 gtgacattca aggatgtggc tgttgtcttc accaaggaag agctggcact attggataaa
      121 gcccagataa acctgtacca agatgtgatg ctggaaaact tcaggaacct catgttagtg
      181 agagacggga ttaaaaacaa cattttgaat cttcaggcaa aggggttaag ttacctttcg
      241 caagaagtgc ttcattgctg gcagatttgg aaacaaagga tccgggattt aactgtgagt
      301 caggattata tcgtgaacct tcaagaagag tgttccccac atttagaaga tgtttccctc
      361 agtgaagagt gggcaggcat ttctcttcag atttctgaaa atgaaaacta tgtagtaaat
      421 gccattatca aaaatcaaga tatcacagca tggcaaagcc tgacacaggt tcttacccca
      481 gaatcgtgga ggaaagccaa cataatgacc gagccccaga actctcaggg aagatataag
      541 ggaatttaca tggaagagaa attgtacaga cgtgctcagc atgatgacag cctcagttgg
      601 acctcatgtg atcatcatga gtcccaagaa tgtaaaggag aggaccctgg tagacatccc
      661 agctgtggga aaaacttggg tatgaaatca acggttgaaa aacgtaatgc ggcccatgta
      721 ttaccacagc ctttcccatg taataactgt ggggtggcct ttgcagatga tacagatcct
      781 catgtccatc acagcactca cctaggagaa aaatcttata aatgtgacca gtatggaaag
      841 aactttagtc agagccaaga tcttatcgtt cattgtaaaa ctcactctgg caagactccc
      901 tatgaattcc acgaatggcc tatgggctgc aaacagagct cagaccttcc cagatatcag
      961 aaagtctcct caggagacaa accctacaaa tgtaaagaat gtggcaaggg cttcaggcgc
     1021 agctcttccc ttcacaacca tcatcgagtc cacacagggg agatgcccta caaatgcgat
     1081 gaatgtggga aagggtttgg atttaggtca cttctttgta ttcatcaggg agtacacaca
     1141 gggaaaaagc cctataaatg tgaagagtgt gggaagggct ttgatcagag ctccaacctt
     1201 cttgtccatc agagagtcca cactggagag aagccctaca aatgcagtga gtgtggcaag
     1261 tgctttagtt caagctccgt tcttcaagtc cactggaggt ttcacacagg ggagaaacca
     1321 tataggtgtg gtgagtgtgg aaagggcttc agccaatgta cacaccttca cattcaccag
     1381 agagtccaca caggggagaa accatacaaa tgcaatgtgt gtggaaagga ttttgcgtat
     1441 agctctgttc ttcacactca tcagagagtt cacactggag aaaaaccata taaatgtgaa
     1501 gtgtgtggaa agtgcttcag ttacagttca tattttcact tacatcaaag agatcacatc
     1561 agagagaaac catataaatg tgatgagtgt ggtaaaggct tcagccggaa ttcagatctt
     1621 aatgttcacc tcagagtcca cacaggagag aggccctata agtgtaaggc atgtggtaag
     1681 ggcttcagtc gtaattcata cctccttgcc catcagagag tgcatataga tgagacacag
     1741 tacacacact gtgagcgtgg aaaggacctt ctgactcatc aaagactaca tgagcagaga
     1801 gaaacattat tgccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     1861 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1921 atttcttggc tttatatatc ttgtggaaag gacgattgta cggtatttgg ctgaattat
//
