LOCUS       sequence-286221-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3226
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(758..782)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             790..831
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgggcctaat ctggctgcta
       61 ctgctcagcc tgctggagcc cggctggccc gcagcgggcc ctggggcgcg gttgcggcgc
      121 gatgcgggcg gccgtggcgg cgtctacgag caccttggcg gggcgccccg gcgccgcaag
      181 ctctactgcg ccacgaagta ccacctccag ctgcacccga gcggccgcgt caacggcagc
      241 ctggagaaca gcgcctacag tattttggag ataacggcag tggaggtggg cattgtggcc
      301 atcaggggtc tcttctccgg gcggtacctg gccatgaaca agaggggacg actctatgct
      361 tcggagcact acagcgccga gtgcgagttt gtggagcgga tccacgagct gggctataat
      421 acgtatgcct cccggctgta ccggacggtg tctagtacgc ctggggcccg ccggcagccc
      481 agcgccgaga gactgtggta cgtgtctgtg aacggcaagg gccggccccg caggggcttc
      541 aagacccgcc gcacacagaa gtcctccctg ttcctgcccc gcgtgctgga ccacagggac
      601 cacgagatgg tgcggcagct acagagtggg ctgcccagac cccctggtaa gggggtccag
      661 ccccgacggc ggcggcagaa gcagagcccg gataacctgg agccctctca cgttcaggct
      721 tcgagactgg gctcccagct ggaggccagt gcgcacttgc caactttctt gtacaaagtg
      781 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
      841 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
      901 gactcgtagt gacccagata ccactc
//
