LOCUS       sequence-286224-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3227
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(848..872)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             880..921
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt ggaacagtgg attcgaaagc
       61 tatggcagct cctcatacgg gggagccggc ggctacacgc agtccccggg gggctttgga
      121 tcgcccgcac cttctcaagc cgaaaagaaa tcaagagccc gagcccagca cattgtgccc
      181 tgtactatat ctcagctgct ttctgccact ttggttgatg aagtgttcag aattgggaat
      241 gttgagattt cacaggtcac tattgtgggg atcatcagac atgcagagaa ggctccaacc
      301 aacattgttt acaaaataga tgacatgaca gctgcaccca tggacgttcg ccagtgggtt
      361 gacacagatg acaccagcag tgaaaacact gtggttcctc cagaaacata tgtgaaagtg
      421 gcaggccacc tgagatcttt tcagaacaaa aagagcctgg tagcctttaa gatcatgccc
      481 ctggaggata tgaatgagtt caccacacat attctggaag tgatcaatgc acacatggta
      541 ctaagcaaag ccaacagcca gccctcagca gggagagcac ctatcagcaa tccaggaatg
      601 agtgaagcag ggaactttgg tgggaatagc ttcatgccag caaatggcct cactgtggcc
      661 caaaaccagg tgttgaattt gattaaggct tgtccaagac ctgaagggtt gaactttcag
      721 gatctcaaga accagctgaa acacatgtct gtatcctcaa tcaagcaagc tgtggatttt
      781 ctgagcaatg aggggcacat ctattctact gtggatgatg accattttaa atccacagat
      841 gcagaatacc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
      901 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
      961 tcttggcttt atatatcttg tggaaaggac gacgtggatg aggtgggcct caaaat
//
