LOCUS       sequence-286227-         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3228
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(430..441)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(518..542)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             550..591
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga gcgataacga tgacatcgag
       61 gtggagagcg acgaagagca accgaggttt caatctgcgg ctgacaaacg ggctcatcat
      121 aatgcactgg aacgaaaacg tagggaccac atcaaagaca gctttcacag tttgcgggac
      181 tcagtcccat cactccaagg agagaaggca tcccgggccc aaatcctaga caaagccaca
      241 gaatatatcc agtatatgcg aaggaaaaac cacacacacc agcaagatat tgacgacctc
      301 aagcggcaga atgctcttct ggagcagcaa gtccgtgcac tggagaaggc gaggtcaagt
      361 gcccaactgc agaccaacta cccctcctca gacaacagcc tctacaccaa cgccaagggc
      421 agcaccatct ctgccttcga tgggggctcg gactccagct cggagtctga gcctgaagag
      481 ccccaaagca ggaagaagct ccggatggag gccagctacc caactttctt gtacaaagtg
      541 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
      601 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
      661 gaccttcttc ggggacggaa gttaca
//
