LOCUS       sequence-286236-        4061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3231
FEATURES             Location/Qualifiers
     source          1..4061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3893..3917)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3925..3966
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgcataagaa gagggttgaa
       61 gagggggagg cctcagactt ctcgctggcc tgggattcct ccgtgacagc agcaggaggc
      121 ctagaaggag agccagagtg cgatcagaaa accagccgtg cgctggaaga caggaacagc
      181 gtgacaagtc aagaggagag aaatgaggat gatgaagaca tggaggatga atcaatttac
      241 acctgcgatc actgtcagca ggacttcgag tctctggcag acctgacgga ccaccgggcc
      301 caccgctgtc ctggagatgg tgatgacgac ccacaactct cctgggtggc ctcgtctccc
      361 tccagcaagg atgttgcgtc acccacgcag atgatcggag atggttgtga cctcggcctc
      421 ggcgaggagg aagggggcac gggcctgcca tacccttgcc agttctgcga caagtccttc
      481 atccgcttga gctacttgaa gaggcacgag cagatccaca gcgacaagct gccgttcaag
      541 tgcacctact gcagccgcct cttcaagcac aagaggagcc gcgaccggca catcaagctg
      601 catacgggcg acaagaagta tcactgccac gagtgcgagg cagccttctc ccgcagcgac
      661 cacctcaaga tccacctgaa gacccacagc tccagcaagc ccttcaagtg cactgtgtgc
      721 aagcgcggct tctcctccac cagctcgctg cagagccaca tgcaggccca caaaaagaac
      781 aaggagcatc tggccaagtc ggagaaggaa gccaagaagg acgacttcat gtgcgactac
      841 tgcgaggaca ccttcagcca gacggaggag ctggagaagc acgtgctcac ccgccacccg
      901 cagctgtccg agaaggcgga cctgcagtgc attcactgcc ctgaggtctt cgtcgacgag
      961 aacacactgc tcgcccatat ccaccaagcc cacgccaacc agaaacacaa gtgccccatg
     1021 tgccctgagc agttctcctc agtggaaggt gtctactgcc acctggacag ccaccggcag
     1081 cccgactcca gcaaccacag tgtcagtccc gaccctgtac tgggcagcgt ggcctccatg
     1141 agcagcgcca cacccgactc cagcgcctct gtggagcgtg gctccacccc ggactccacc
     1201 ttgaagccgc tgcgggggca gaagaagatg cgggatgacg ggcagggctg gaccaaggtg
     1261 gtctatagct gcccctattg ttccaagcgg gactttaaca gcctggccgt gctggagatc
     1321 cacctgaaga ccatccacgc ggacaagccc cagcagagcc acacatgtca gatctgcctg
     1381 gactccatgc ccaccctcta caacctcaac gagcacgttc gcaagctgca caagaaccat
     1441 gcctaccctg tgatgcagtt tggcaacatc tctgccttcc actgcaacta ctgccccgag
     1501 atgttcgccg acatcaatag cctgcaggag cacatccgcg tctcccactg cggccccaac
     1561 gccaacccct ctgacggtaa taatgctttc ttctgcaacc agtgctccat gggtttcctt
     1621 actgagtcct ccctcaccga gcacatccag caggcccact gcagtgtggg cagtgccaaa
     1681 ctagagtctc cggtggtgca gcccacgcag tccttcatgg aggtctattc ctgcccctac
     1741 tgcaccaact cccccatctt tggctccatc ctgaaactca ccaagcacat caaggagaac
     1801 cacaagaaca ttccactggc ccacagcaag aagtccaagg ccgagcagag cccagtctcg
     1861 tccgatgtgg aggtgtcttc cccgaagcgg cagcggctct cagcaagcgc caactccatc
     1921 tccaatgggg agtatccttg taatcaatgc gacctcaagt tctccaactt tgagagcttc
     1981 cagacccacc tgaagctgca cctggagctg ctgctgcgga agcaagcgtg cccccagtgc
     2041 aaagaggact ttgactccca ggagtccctc ctgcagcacc tgacagtgca ttacatgacc
     2101 acgtcgaccc actatgtgtg cgagagctgc gacaagcaat tttcctcggt ggatgacctg
     2161 cagaagcacc tgctggacat gcacaccttt gtgttgtacc actgcaccct gtgtcaggag
     2221 gtcttcgact ccaaggtgtc catccaggtg cacctggcgg tgaagcacag caatgagaag
     2281 aagatgtacc gctgcacggc ctgcaactgg gacttccgca aggaggctga cctgcaggtg
     2341 cacgtcaaac acagccacct gggcaacccg gccaaggctc acaagtgcat cttctgtggg
     2401 gagaccttca gcaccgaggt ggagctgcag tgccacatca ccacacacag caagaagtat
     2461 aactgtaagt tctgcagcaa ggccttccac gccatcatcc tgctggagaa gcacctgcgg
     2521 gagaagcact gtgtgtttga tgctgcgacc gagaacggca cggccaatgg ggtaccccca
     2581 atggccacca agaaagctga gcctgctgac ctgcagggca tgctgcttaa gaaccctgag
     2641 gcacctaaca gccatgaggc cagcgaggat gacgtggacg cgtcggagcc catgtacggc
     2701 tgtgacatct gcggggcggc ctacaccatg gaggtgctgc tgcagaatca ccggctgcgg
     2761 gaccacaata tccggccggg cgaggatgat ggctcacgca agaaggctga gtttatcaag
     2821 ggcagtcaca agtgcaacgt ttgttcacgg actttcttct cggagaacgg gctacgggag
     2881 cacctgcaga cgcaccgggg ccctgccaag cactacatgt gtcccatctg tggtgagcgc
     2941 ttcccttcgc tgctgacgct caccgaacac aaggtgaccc acagcaagag cctggacacg
     3001 ggcacctgtc gcatctgcaa gatgcccctg cagagcgagg aggagtttat tgagcactgc
     3061 cagatgcacc ctgacctgcg caactcactc acgggcttcc gctgtgtggt ctgcatgcag
     3121 acagtcactt ccacgcttga gctcaagatc catggcacct tccacatgca gaagctggcg
     3181 ggcagctcag cggcgtcctc ccccaatggc caggggctgc agaagctcta caagtgcgcc
     3241 ctgtgcctca aggagttccg cagcaagcag gacctggtga agcttgacgt caatgggctg
     3301 ccctacggcc tctgcgccgg ctgcatggcc cgcagcgcca acggacaggt gggtggcctg
     3361 gccccgcccg agcccgccga ccggccctgt gccggcctcc gttgccccga gtgcagtgtc
     3421 aagtttgaga gtgccgaaga cctggagagc cacatgcagg tggaccaccg tgacctcacg
     3481 ccggagacca gtgggccccg gaaaggcacc cagacatcgc cagtgccccg gaaaaagaca
     3541 taccagtgca tcaagtgcca gatgaccttc gagaacgaga gagagatcca aatccacgtt
     3601 gccaaccaca tgattgagga aggcatcaac cacgagtgta agctgtgcaa ccagatgttc
     3661 gactccccgg ccaagctcct ctgtcacctc attgagcaca gcttcgaggg catgggcggc
     3721 accttcaaat gccccgtgtg tttcacagtc ttcgtccagg ccaacaagtt gcagcagcac
     3781 atctttgccg tgcacgggca ggaggacaag atctacgact gctcacagtg ccctcagaag
     3841 ttcttcttcc agaccgagct gcagaaccac acgatgagcc agcacgcaca gttgccaact
     3901 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     3961 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     4021 tcttgtggaa aggacgagcc tactcaagcg caagcgaatg c
//
