LOCUS       sequence-286239-        1247 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3232
FEATURES             Location/Qualifiers
     source          1..1247
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1079..1103)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1111..1152
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt gcactaaaat ggaacagccc
       61 ttctaccacg acgactcata cacagctacg ggatacggcc gggcccctgg tggcctctct
      121 ctacacgact acaaactcct gaaaccgagc ctggcggtca acctggccga cccctaccgg
      181 agtctcaaag cgcctggggc tcgcggaccc ggcccagagg gcggcggtgg cggcagctac
      241 ttttctggtc agggctcgga caccggcgcg tctctcaagc tcgcctcttc ggagctggaa
      301 cgcctgattg tccccaacag caacggcgtg atcacgacga cgcctacacc cccgggacag
      361 tacttttacc cccgcggggg tggcagcggt ggaggtgcag ggggcgcagg gggcggcgtc
      421 accgaggagc aggagggctt cgccgacggc tttgtcaaag ccctggacga tctgcacaag
      481 atgaaccacg tgacaccccc caacgtgtcc ctgggcgcta ccggggggcc cccggctggg
      541 cccgggggcg tctacgccgg cccggagcca cctcccgttt acaccaacct cagcagctac
      601 tccccagcct ctgcgtcctc gggaggcgcc ggggctgccg tcgggaccgg gagctcgtac
      661 ccgacgacca ccatcagcta cctcccacac gcgccgccct tcgccggtgg ccacccggcg
      721 cagctgggct tgggccgcgg cgcctccacc ttcaaggagg aaccgcagac cgtgccggag
      781 gcgcgcagcc gggacgccac gccgccggtg tcccccatca acatggaaga ccaagagcgc
      841 atcaaagtgg agcgcaagcg gctgcggaac cggctggcgg ccaccaagtg ccggaagcgg
      901 aagctggagc gcatcgcgcg cctggaggac aaggtgaaga cgctcaaggc cgagaacgcg
      961 gggctgtcga gtaccgccgg cctcctccgg gagcaggtgg cccagctcaa acagaaggtc
     1021 atgacccacg tcagcaacgg ctgtcagctg ctgcttgggg tcaagggaca cgccttctgc
     1081 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     1141 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1201 tatatatctt gtggaaagga cgagactgat ggctcgcgag ctcgctt
//
