LOCUS       sequence-286248-        1736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3235
FEATURES             Location/Qualifiers
     source          1..1736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1568..1592)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1600..1641
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggctctttc tcagggtcta
       61 ctgacattca gggatgtggc catagaattc tctcaggagg agtggaaatg cctggaccct
      121 gctcagagga ctctatacag agacgtgatg ctggagaatt ataggaacct ggtctccctg
      181 gatatctctt ccaaatgcat gatgaataca ttgtcatcaa cagggcaagg caatacagaa
      241 gtgatccaca cagggacatt gcagagacaa gcaagttatc acattggagc attttgctcc
      301 caggaaattg agaaagacat tcatgacttt gtgtttcagt ggcaagaaga tgaaacaaat
      361 gaccatgaag cacccatgac agaaataaaa aagttgacta gtagtacaga ccgatatgat
      421 caaaggcatg ctggaaacaa gcctattaaa ggtcagcttg aatcaagatt tcatttgcat
      481 ttgcgaagac ataggagaat tcatactgga gagaaacctt acaaatgtga agaatgtgag
      541 aaagttttca gttgcaaatc acatcttgaa atacatagga taattcatac tggagagaaa
      601 ccatacaaat gtaaggtttg cgacaaggct tttaagcatg attcacacct ggcaaaacat
      661 actagaattc acaggggaga caaacattac acatgtaatg aatgtggcaa ggtttttgat
      721 caaaaagcaa cccttgcatg tcatcataga agtcatactg gagagaaacc ttataagtgt
      781 aatgagtgtg gcaagacctt tagtcagaca tcacaccttg tgtaccatca tagactgcat
      841 actggagaga aaccttacaa atgtaatgag tgtggcaaga ccttcgctcg aaattcagtc
      901 ctcgtaattc ataaggcagt tcatactgca gagaaaccct ataagtgtaa tgaatgtggc
      961 aaggttttta agcaacgagc aactcttgca ggacatcgta gagttcacac tggagagaaa
     1021 ccttacagat gtgaagaatg cgacaaagtt ttcagtcgca aatcacatct tgaaagacat
     1081 aggaggattc atactggaga gaaaccatac aaatgtaagg tttgtgacaa ggctttccgg
     1141 agtgattcac gtcttgcaga acatcagaga gttcatactg gagagagacc ttacacatgt
     1201 aatgaatgtg gcaaggtttt tagtacaaaa gcgtacctcg catgtcatca aaaacttcat
     1261 actggagaga aactttacga atgtgaagaa tgtgacaaag tttacattcg caaatcacac
     1321 cttgaaagac ataggaggat tcatacagga gagaaacctc acaagtgtgg tgattgtggt
     1381 aaagccttta attcaccttc acaccttatt aggcatcaga gaatccatac tggacagaaa
     1441 tcttacaaat gtcatcagtg tggcaaggtc tttagtctga ggtcactcct tgcagaacat
     1501 cagaaaattc cttttggaga caattgtttc aagtgcaatg agtatagcaa accatcaagc
     1561 attaatttgc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1621 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1681 tcttggcttt atatatcttg tggaaaggac gacgcggtat gattagcata tccgat
//
