LOCUS       sequence-286251-        2810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3236
FEATURES             Location/Qualifiers
     source          1..2810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             2578..2586
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(2642..2666)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2674..2715
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggagaacga aaaggaaaat
       61 ctcttttgtg agccacataa aaggggacta atgaaaacac ctctgaaaga atccaccaca
      121 gcaaatatcg tgttggcaga gatccagcct gactttggcc ctttaaccac acctaccaag
      181 cccaaggaag gctctcaggg agagccgtgg acaccgacag ccaacctgaa aatgctcatc
      241 agtgctgtga gccctgagat ccgcaacaga gatcagaaaa ggggtttgtt tgacaacaga
      301 agtggattac ctgaggccaa agactgtata cacgaacact tatctggaga tgaatttgag
      361 aaatcccaac caagtcgaaa agagaaaagt ttaggattat tgtgtcataa gttcttagca
      421 cgatatccta attatcccaa ccctgctgtg aataatgaca tctgccttga cgaagtggca
      481 gaggaactta atgttgaacg tcgacgcatt tacgatatcg tgaacgtcct agagagttta
      541 catatggtga gccgcctcgc caaaaacagg tacacttggc acgggcgaca caatctcaac
      601 aaaacccttg gcaccttgaa gagcatcggg gaggagaata agtacgccga gcagattatg
      661 atgatcaaaa agaaagaata tgagcaagag tttgacttta ttaagagtta cagtatagag
      721 gatcatatca tcaaatcaaa cactggccca aatggacacc cagacatgtg ttttgtggaa
      781 ctccctggag tggaatttcg ggcagcttct gtaaacagcc gcaaagacaa gtctttaagg
      841 gtaatgagcc agaaatttgt gatgctgttt ttggtgtcaa cgcctcagat agtaagccta
      901 gaagttgctg ccaagatttt aattggggag gaccatgtgg aagatttgga taaaagcaag
      961 tttaaaacaa aaattaggag gttgtatgat atagctaatg ttctgagtag cctggatctt
     1021 atcaagaaag ttcatgttac agaggaaaga ggccgaaaac cagctttcaa atggaccggc
     1081 ccagaaatca gtccaaatac cagtggctcc agcccagtca ttcattttac tccctctgat
     1141 ttggaggtga gacggtcttc aaaagagaac tgtgccaaaa acctcttttc cacacgtggg
     1201 aaaccaaact ttactcgaca cccatctctt atcaaattgg taaagagtat agaaagtgat
     1261 cggagaaaga taaattctgc gcccagtagc cctatcaaga ccaacaaagc tgagagttct
     1321 cagaattctg cacccttccc aagtaaaatg gctcagctcg cagctatttg taaaatgcag
     1381 ttagaagagc aatcaagtga atccagacag aaagtgaaag tacagctggc aagatctgga
     1441 ccctgcaaac cagtagcccc tctggacccc ccagtgaatg ctgagatgga gctgacagca
     1501 ccgtccctca tccagcccct gggaatggtt cccctgatcc ccagcccctt gtcatcagca
     1561 gtgcccctga tcctacctca ggccccttca ggcccatcct atgccatcta cctgcagccc
     1621 actcaagccc accaaagtgt gaccccaccc caaggcctga gcccaacggt gtgcaccacc
     1681 cactcttcta aagctactgg ctcaaaagac tccacagatg ccaccactga gaaggcagcc
     1741 aatgatacct caaaggccag tgcctctacc aggcctggaa gcttgctgcc agcaccagag
     1801 aggcaagggg caaagagccg aaccagggag ccagctggag aaagaggctc aaagagggca
     1861 agcatgctcg aggacagtgg ttccaaaaag aaatttaaag aggacctaaa aggacttgaa
     1921 aatgtctccg caaccttgtt cccatcagga tacctaatcc ctctcacgca gtgctcatcc
     1981 ctgggggcag agtccatttt gtctggtaaa gaaaactcaa gtgctctttc cccaaaccac
     2041 aggatttaca gctccccaat tgcaggtgtt attccagtga catcatctga actcactgct
     2101 gttaattttc cctcttttca tgtaacaccg ttgaagctaa tggtctcacc aacttccgtg
     2161 gcagccgtac ctgtcgggaa cagcccggct ctcgcttcaa gccaccctgt tcccatccag
     2221 aacccaagct cagccattgt aaacttcacc ctgcagcacc tgggactcat ctcacccaat
     2281 gtgcagttgt ctgccagccc tgggtctgga atcgttcctg tgtctccaag aatagagtct
     2341 gttaatgtcg caccagaaaa tgcaggcact cagcaaggaa gggccaccaa ctatgactca
     2401 ccagtcccag gccagagcca gccaaatgga caatcagttg ctgtgacagg ggcacaacag
     2461 cctgttcctg tgacacccaa agggtcacaa ttagtggccg aaagtttctt ccgtacccca
     2521 ggtggaccca ccaagccaac cagctcatcc tgcatggatt ttgagggtgc taataaaacc
     2581 tccttaggaa ctctctttgt cccacagcga aaactggaag tctcaacaga ggatgtccat
     2641 ttgccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     2701 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     2761 ctttatatat cttgtggaaa ggacgacgca aatgtgtgca tcgcacgtct
//
