LOCUS       sequence-286257-        1337 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3238
FEATURES             Location/Qualifiers
     source          1..1337
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             280..300
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    complement(1169..1193)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1201..1242
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcgaactc ggcaaataca
       61 aacaccgtgc ctaaattata cagatctgtg attgaagatg tcattaatga tgtgagagac
      121 atctttctgg atgatggagt ggatgaacaa gtactgatgg aactaaaaac tttatgggaa
      181 aacaaactaa tgcagtccag ggcagtagat ggatttcatt cagaagagca gcagcttcta
      241 ctgcaagttc aacagcagca tcaaccccag cagcagcagc atcaccacca tcaccatcat
      301 cagcaagctc agcctcagca gacagtacct cagcaagcgc agacccagca ggttcttatt
      361 cctgcatcac agcaagccac agcaccacaa gttattgttc cagattctaa gttgatacag
      421 catatgaatg catcaaacat gagtgctgct gctacagctg ctaccttagc actccctgca
      481 ggtgtgactc ctgttcagca gatattaaca aattcaggcc agcttcttca ggtggtcaga
      541 gcagccaatg gtgcccaata tatctttcag cctcagcagt cagtggttct acaacaacag
      601 gttataccac aaatgcagcc tggtggagta caagctcctg ttatacagca ggtgctggct
      661 cctcttcctg gagggatttc accacagaca ggtgtcatca tccagcctca gcaaatctta
      721 tttacaggaa ataagactca agttatacct acgacagtgg cagcacctac accagcccaa
      781 gcacagataa ctgcaactgg ccagcagcaa ccgcaggccc agcctgctca aacacaagct
      841 ccattggtct tacaagttga tggaactggg gatacatcat ctgaagaaga tgaagatgaa
      901 gaagaagact atgatgatga tgaggaggaa gacaaagaga aagatggagc tgaagatggg
      961 caggtggaag aagagcccct caatagtgaa gatgatgtga gtgatgagga aggacaggaa
     1021 ctctttgaca cagaaaatgt tgttgtatgc caatatgata agatacacag aagtaaaaac
     1081 aaatggaaat ttcatctcaa ggatggcatt atgaatctta atggaagaga ttatatattt
     1141 tccaaagcca ttggagatgc agaatggttg ccaactttct tgtacaaagt ggttgatatc
     1201 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1261 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaagtcagt
     1321 tctcctaagg accgctc
//
