LOCUS       sequence-286260-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3239
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(848..872)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             880..921
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgagctcgta ctttgtaaac
       61 tccttctcgg ggcgttatcc aaatggcccg gactatcagt tgctaaatta tggcagtggc
      121 agctctctga gcggctctta cagggatccc gctgccatgc acaccggctc ttacggctac
      181 aattacaatg ggatggacct cagcgtcaac cgctcctcgg cctcctccag ccactttggg
      241 gcggtgggcg agagctcgcg cgccttcccc gcgcccgccc aggagccccg cttcaggcaa
      301 gcggcttcga gctgctccct gtcctcgccc gagtccctgc cctgcaccaa cggcgacagc
      361 cacggcgcca agccctctgc ttcgtccccc tccgaccagg cgacctcagc cagctccagc
      421 gccaatttca ccgaaataga cgaggccagc gcgtcctcgg agcctgagga agcggcaagc
      481 cagctaagca gccccagcct agctcgggcg cagccagagc ccatggccac ctccacagcc
      541 gcgcccgagg ggcagactcc gcaaatattc ccctggatga ggaagcttca catcagccat
      601 gatatgaccg ggccggacgg gaaaagggcc cggaccgcgt atacccgcta ccagaccctg
      661 gagctggaaa aggagttcca cttcaaccgc tacctgaccc ggcgacggcg catcgagatc
      721 gcccacgcac tctgcctgtc cgagcgccag atcaagatct ggttccagaa ccggcgcatg
      781 aagtggaaga aggacaacaa attgaaaagt atgagcctgg ctacagctgg cagcgccttc
      841 cagcccttgc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
      901 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
      961 tcttggcttt atatatcttg tggaaaggac gatcccagcg gtgccatatt caagaa
//
