LOCUS       sequence-286263-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3240
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(806..830)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             838..879
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc tctggcaaaa acccacagct
       61 ccggagcaag ccccagcccc ggcccggcca taccagggcg tccgtgtgaa ggagccagtg
      121 aaggaactgc tgaggaggaa gcgaggccac gccagcagtg gggcagcacc tgcacctacg
      181 gcggtggtgc tgccccatca gcccctggcg acctacacca cagtgggtcc ttcctgcctg
      241 gacatggaag gttctgtgtc tgcagtgaca gaggaggctg ccctgtgtgc cggctggctc
      301 tcccagccca ccccggccac cctgcagccc ctggccccat ggacacctta caccgagtat
      361 gtgccccatg aagctgtcag ctgcccctac tcagctgaca tgtatgtgca gcccgtgtgc
      421 cccagctaca cggtggtggg gccctcctca gtgttgacct atgcctctcc gccactcatc
      481 accaatgtca cgacaagaag ctccgccacg cccgcagtgg ggcccccgct ggagggccca
      541 gagcaccagg cacccctcac ctatttcccg tggcctcagc ccctttccac actacccacc
      601 tccaccctgc agtaccagcc tccggcccca gccctacctg ggccccagtt tgtccagctc
      661 cccatctcta tcccagagcc agtccttcag gacatggaag accccagaag agccgccagc
      721 tcgttgacca tcgacaagct gcttttggag gaagaggata gcgacgccta tgcgcttaac
      781 cacactctct ctgtggaagg cttttaccca actttcttgt acaaagtggt tgatatcggt
      841 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
      901 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga tcaggtgtta
      961 gatccggctg ggtt
//
