LOCUS       sequence-286266-        1793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3241
FEATURES             Location/Qualifiers
     source          1..1793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1625..1649)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1657..1698
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc gcaggaattc cagtctcagt
       61 ttccagatgg agcgacccct cgaggagcaa gtccagagca agtggtcgtc tagtcaaggc
      121 cgcacaggaa caggagggtc tgatgtcctc cagatgcaga acagtgaaca ccatggacaa
      181 agcatcaaga ctcaaactga ctccatctcc cttgaggatg tggctgtgaa cttcaccctg
      241 gaggagtggg ctttgctgga tcctggccag aggaatatct acagagatgt gatgcgggca
      301 accttcaaga acctggcctg tataggggaa aaatggaaag accaggatat tgaagatgaa
      361 cacaaaaacc agggaagaaa tctaagaagt cctatggttg aagcactctg tgaaaataaa
      421 gaagattgtc catgtggaaa aagcactagc cagattcctg atcttaatac gaacctggaa
      481 actcctactg gattaaaacc atgtgactgc agtgtgtgtg gggaagtctt catgcatcag
      541 gtctccctta ataggcacat gagatctcac actgaacaga aaccaaatga gtgtcacgaa
      601 tatggagaga agccacataa atgcaaagaa tgtgggaaaa ccttcactcg cagctccagt
      661 attcgaaccc atgaaagaat tcacactgga gagaaaccct atgaatgtaa ggaatgtggc
      721 aaagccttcg catttctctt ttcctttcga aaccatataa gaattcatac tggagagaca
      781 ccctatgaat gtaaggaatg tgggaaggca ttcagatatc tcactgctct tcggcgccat
      841 gaaaaaaatc acactggaga gaaaccctac aaatgtaaac agtgtggaaa agcctttata
      901 tattaccagc cttttctaac ccatgaaagg actcacactg gagagaaacc ttatgaatgt
      961 aagcaatgtg ggaaagcctt cagttgtccc acgtacttac ggagtcatga gaaaactcat
     1021 actggagaga aaccttttgt atgtagggaa tgtgggagag ccttcttttc tcactcaagc
     1081 cttcgaaaac acgtgaaaac ccacaccgga gttcaacctt atacatgtaa gaaatgtggg
     1141 gaagccttca agtcatctag ttcctgtgaa gtgcacgaaa gaactcattt tggagaaaaa
     1201 ccctatgaat gtaaacaatg tggtaaagcc ttcaattctt caagttacct tcagttgcac
     1261 gaaagagttc acactggcga gaaaacttac gaatgtaaag aatgtggtaa agcctttctt
     1321 tattccactc actttcgaat ccatgaaaga acccatacta gagagaaacc ctatgaatgc
     1381 aaacagtgtg gtcgggtctt catttacttc agtcaccttc gaaggcacga aagaagtcac
     1441 actggagtga aaccatgtga gtgtaagcag tgtggcaaag ctttcacttg tttaaattcc
     1501 ctgaaagtac acaaaagaat tcatactgga gaaagaccct ttcagtgtag acaatgtggt
     1561 aaagccttca gttactcaaa gtctttgcac gtgcacgaaa ggactcatag tagacagaag
     1621 ccctacccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     1681 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1741 tggctttata tatcttgtgg aaaggacgac acaccatctc tttactgctc cga
//
