LOCUS       sequence-286272-        2333 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3243
FEATURES             Location/Qualifiers
     source          1..2333
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggactgtt gacattcagg gatgtggccg tagaattctc tttggaggag
       61 tgggaacacc tggaaccagc tcagaagaat ttgtatcagg atgtgatgtt agaaaactac
      121 agaaacctgg tctctctggg tcttgttgtc tctaagccgg acctgatcac ctttttggaa
      181 caaaggaaag agccttggaa tgtgaagagt gaggagacag tagccatcca gccagatgtg
      241 ttttcgcatt ataacaagga cctgttgaca gagcactgca cagaagcttc attccaaaaa
      301 gtgatatcga ggagacatgg gagctgtgat cttgagaatt tacatttaag aaaaaggtgg
      361 aaaagggagg agtgtgaagg gcacaatgga tgttatgatg aaaagacttt taaatatgat
      421 caatttgatg aatcctctgt tgaaagtttg tttcaccagc aaatactttc ttcttgtgcc
      481 aaaagctata actttgatca atataggaag gtctttactc attcatcatt gcttaatcaa
      541 caagaggaaa tagatatttg gggaaaacat cacatatatg ataaaacttc agtgttattt
      601 aggcaggtct ctactctaaa tagttaccga aatgttttta ttggagagaa aaattatcat
      661 tgcaataatt ctgaaaaaac cttgaaccaa agctcaagcc ctaaaaatca tcaggaaaat
      721 tattttctag aaaaacaata caaatgtaaa gaatttgagg aagtctttct tcagagtatg
      781 catgggcaag agaaacaaga acagtcttac aaatgtaata aatgtgtaga agtttgtacc
      841 cagtcattaa aacatattca acatcagacc atccatatca gagaaaactc atatagctat
      901 aacaaatatg ataaagatct tagtcagtca tcaaatctta gaaagcagat aatccataat
      961 gaagagaaac catacaaatg tgaaaaatgt ggggatagct taaaccatag tttgcacctt
     1021 actcaacatc agatcattcc taccgaagag aaaccctgta aatggaaaga atgtggcaag
     1081 gtctttaacc ttaactgtag tttatacctt actaaacagc agcaaattga tactggagaa
     1141 aacctttaca aatgtaaagc atgtagcaaa tcttttactc gttcctccaa tcttattgtg
     1201 catcagagaa ttcacactgg agagaaacca tacaaatgta aagaatgtgg caaagccttt
     1261 cgctgtagtt cataccttac taaacataag cgaattcata ctggagagaa accttataaa
     1321 tgtaaagaat gtggaaaagc ttttaaccgt agttcatgcc ttactcaaca tcagacaact
     1381 catacaggag aaaaacttta caaatgtaaa gtatgtagca aatcttatgc tcgttcttca
     1441 aatcttatta tgcatcagag agttcatact ggagagaagc cttataaatg taaagaatgt
     1501 ggcaaagtct ttagccgtag ttcttgcctt actcaacatc ggaaaattca tactggagaa
     1561 aatctttaca aatgcaaagt atgtgctaaa ccttttactt gtttctcaaa tcttattgtg
     1621 catgagagaa ttcatactgg agagaaaccc tataaatgta aagaatgtgg caaagccttt
     1681 ccttatagtt cacaccttat tcgacatcat cgaattcata ctggagaaaa accatacaaa
     1741 tgtaaagcat gtagcaaatc ttttagtgac tcctcaggtc ttactgtgca tcggcgaact
     1801 catactggag agaaacccta tacatgtaaa gaatgtggca aagcctttag ttatagttca
     1861 gatgttattc agcatcggag aattcatact ggccagagac cctacaaatg tgaagaatgt
     1921 ggcaaagcct tcaactatag gtcatacctc actacacatc agagaagtca tactggagag
     1981 agaccctaca aatgtgaaga atgtggcaaa gccttcaact ctaggtcata cctcactaca
     2041 catcggagaa gacatactgg agagagaccc tacaaatgtg atgaatgtgg taaagccttc
     2101 agctataggt catacctcac tacacatcgg agaagtcata gtggagagag accctacaaa
     2161 tgtgaagaat gtggcaaagc ctttaactct aggtcatacc tcattgcaca tcagagaagt
     2221 catactagag aaaaacttta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     2281 tggctttata tatcttgtgg aaaggacgat cgaaagcacg ctgcggagcg tga
//
