LOCUS       sequence-286275-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3244
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(770..794)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             802..843
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc aagctgtagt gcccttgaac
       61 aagatgacag ccatctcacc agaacctcaa actctggcct cgactgaaca aaatgaggtc
      121 ccaagagtgg ttacttctgg ggaacaagaa gctattttaa gaggaaatgc tgctgatgca
      181 gagtctttca gacagaggtt taggtggttt tgttactcag aagtagctgg acccaggaaa
      241 gctctgagtc aactctggga gctctgcaat cagtggctga gaccagacat tcacacgaaa
      301 gaacagattt tagagcttct ggtgtttgag cagttcctga ccattttgcc tggggagatc
      361 aggatttggg taaagtcaca acatcctgag agtagtgagg aagtggtgac cctaatagaa
      421 gatttgaccc agatgcttga agaaaaagat ccagtctctc aagattctac tgtttcccaa
      481 gaggagaact caaaagagga taaaatggtc actgtttgtc ccaatactga gtcctgtgaa
      541 tctataacat tgaaggatgt agctgtgaac ttttcaagag gagagtggaa gaagctggag
      601 ccttttcaaa aggagctata taaggaagtg ctactggaaa acctcaggaa cctagaattt
      661 ctggactttc cagtttcaaa attagagttg atttcccagc taaagtgggt tgaattgcca
      721 tggctgctgg aagaagtctc aaaaagctcc cgactaggaa gtgtgatttg cccaactttc
      781 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
      841 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
      901 tgtggaaagg acgaactaat gtcagatacc acctcctc
//
