LOCUS       sequence-286278-         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3245
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(524..548)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             556..597
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga cgacccccaa taaaggaaac
       61 aaggccttga aggtgaagcg ggagccgggt gagaatggca ccagcctgac ggatgaggag
      121 ctggtgacca tgtcggtgcg ggagctgaac cagcacctgc ggggcctgtc caaggaggag
      181 atcgtccagc tgaagcagcg ccggcgcacg ctcaagaacc gcggctacgc tgccagctgc
      241 cgcgtgaagc gggtgacgca gaaggaggag ctggagaagc agaaggcgga gctgcagcag
      301 gaggtggaga agctggcctc agagaacgcc agcatgaagc tggagctcga cgcgctgcgc
      361 tccaagtacg aggcgctgca gaccttcgcc cggacggtgg cccgcagccc cgtggcgcca
      421 gcccggggcc cccttgccgc cggcctgggg cccctcgtcc caggcaaggt ggccgccacc
      481 agcgtcatca caatagtaaa gtccaagacg gatgcccgat cgtacccaac tttcttgtac
      541 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
      601 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      661 aaggacgata tgatcaaaga gggtttgctt cc
//
