LOCUS       sequence-286281-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3246
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(818..842)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             850..891
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt cggcgacggg gcccatcagt
       61 aactattacg tggactcgct catctctcac gacaatgaag acctcctagc gtccaggttt
      121 ccggccaccg gggctcatcc cgccgccgcc agacccagcg gtttggtgcc ggactgtagc
      181 gattttccgt cctgtagctt cgcgcccaag ccggcagtgt tcagcacgtc gtgggcgccc
      241 gtgccctctc agtcgtccgt ggtatatcac ccgtacggcc cccagcccca cctcggcgcc
      301 gacacgcgct acatgcggac ttggctcgag ccgctgtccg gcgccgtctc cttccccagc
      361 ttcccggccg ggggccgtca ctacgccctc aagccggacg cctaccccgg gcgccgcgcg
      421 gactgcggcc caggggaggg ccgcagctac ccggactaca tgtacggctc gcccggggag
      481 ctgcgcgacc gcgccccgca gacactgccc tcgcccgagg cggacgcgct cgccggcagc
      541 aagcacaaag aggagaaggc tgacctggac cccagcaacc ccgtggccaa ctggattcac
      601 gcccgctcca cgaggaagaa gcgctgcccc tacaccaagt accagacgct ggaactggag
      661 aaggagtttc tcttcaatat gtatttaacc agggaccgtc ggtatgaggt ggcccgggtt
      721 ctcaatctca ccgagcggca ggtcaaaatc tggtttcaga atcgaaggat gaagatgaaa
      781 aagatgaata aagagaaaac cgacaaggag cagtcctacc caactttctt gtacaaagtg
      841 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
      901 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
      961 gacgcggcta cgtagctttt tcatga
//
