LOCUS       sequence-286284-        1403 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3247
FEATURES             Location/Qualifiers
     source          1..1403
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1235..1259)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1267..1308
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc cccaactctc cggaggaggt
       61 ggcggcggcg ggggggaccc ggaactctgc gccacggacg agatgatccc cttcaaggac
      121 gagggcgatc ctcagaagga aaagatcttc gccgagatca gtcatcccga agaggaaggc
      181 gatttagctg acatcaagtc ttccttggtg aacgagtctg aaatcatccc ggccagcaac
      241 ggacacgagg tggccagaca agcacaaacc tctcaggagc cctaccacga caaggccaga
      301 gaacaccccg atgacggaaa gcatccagat ggaggcctct acaacaaggg accctcctac
      361 tcgagttatt ccgggtacat aatgatgcca aatatgaata acgacccata catgtcaaat
      421 ggatctcttt ctccacccat cccgagaaca tcaaataaag tgcccgtggt gcagccatcc
      481 catgcggtcc atcctctcac ccccctcatc acttacagtg acgagcactt ttctccagga
      541 tcacacccgt cacacatccc atcagatgtc aactccaaac aaggcatgtc cagacatcct
      601 ccagctcctg atatccctac tttttatccc ttgtctccgg gtggtgttgg acagatcacc
      661 ccacctcttg gctggcaagg tcagcctgta tatcccatca cgggtggatt caggcaaccc
      721 tacccatcct cactgtcagt cgacacttcc atgtccaggt tttcccatca tatgattccc
      781 ggtcctcctg gtccccacac aactggcatc cctcatccag ctattgtaac acctcaggtc
      841 aaacaggaac atccccacac tgacagtgac ctaatgcacg tgaagcctca gcatgaacag
      901 agaaaggagc aggagccaaa aagacctcac attaagaagc ctctgaatgc ttttatgtta
      961 tacatgaaag aaatgagagc gaatgtcgtt gctgagtgta ctctaaaaga aagtgcagct
     1021 atcaaccaga ttcttggcag aaggtggcat gccctctccc gtgaagagca ggctaaatat
     1081 tatgaattag cacggaaaga aagacagcta catatgcagc tttatccagg ctggtctgca
     1141 agagacaatt atggtaagaa aaagaagagg aagagagaga aactacagga atctgcatca
     1201 ggtacaggtc caagaatgac agctgcctac atctgcccaa ctttcttgta caaagtggtt
     1261 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     1321 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     1381 agtacatccg cgtcaccgtc cca
//
