LOCUS       sequence-286287-        2207 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3248
FEATURES             Location/Qualifiers
     source          1..2207
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2039..2063)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2071..2112
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgcaggaggt tgtgctgttg
       61 gatgagagtt caggaccacc aagccagctg ctttggaccc gccaggatac ccagctccct
      121 caggaaagcg ctctattacc tgctccatat cctgccttca ctaaagatgg aagccaagga
      181 aacctgccgc aagcagatat cacactaatg agccaggccc aagagtcagt gacatttgag
      241 gatgtagctg tgaacttcac taacagggag tggcagtgtc tgacctacgc tcaaaggcat
      301 ctctataagg atgtgatgtt ggaaaattat gggaacatgg tatcacttgg atttccattt
      361 cctaaacctc ctttaatctc tcatctggag cgagaagtag acccctgtgt gcaggatcca
      421 caggacaggg agtccctaag ctgctcctac ccagtgtcag ctgacaagat gtggcctgag
      481 aatgaaaagg caagttcaca acaagagatt tttgaaaatg gagaagccta ctggatgaaa
      541 tttaacagtc tcctaaaagt tgattcccgg gatcctaagg ttagagaagt ttgtgttcag
      601 gatgtcaaat tagagaatca atgggaaaca tctataaggg agaaactgag agaagagaaa
      661 gaaggctctg aggaagtgac ctgcaaaaaa ggaaagaacc agaaagtgct tagtaaaaac
      721 ttgaatccaa actcaaaaca tagtcaatgt aataaagttc ttatagcaca gaaactccat
      781 gaatgtgcca ggtgtggcaa aaacttcagt tggcactcag atctaattct ccatgagcaa
      841 attcattctg gtgagaaacc ccatgtgtgt aatgagtgtg ggaaagcatt caagaccaga
      901 aatcagcttt ctatgcaccg gataatccac acaggggaga aaccttttaa ttgcacccag
      961 tgtgggaagg ctttcaacag tagatcagct ctttgccgac ataaaaaaac ccacagtggg
     1021 gagaagcctc acgagtgcag ggactgtggg aaggccttca agaccaggaa ccgtctctgt
     1081 atgcatcagc ttatccacac cggggagaag ccttacaaat gtaactgctg tgggaaggcc
     1141 ttccagttta agcattccct taccatccat ggcagaatcc acactgggga gaagccatat
     1201 gaatgtgagg agtgcgggaa ggccttcagt gggagttcag acctcaccaa acacataaga
     1261 atccacactg gggaacgacc ttatgagtgc agcaagtgtg gaagggcctt cagtcggagc
     1321 tcagacctaa gcaaacacaa acgaatccat actcgggaga aacactatgg gtgtccccag
     1381 tgtggaaaag acttcagcat caaggcagaa ctcaccaaac acagaaggat ccacactgaa
     1441 gagaaacgtt acaggtgtga ggagtgtggg aaagcctttc gtcataactg taagcgcagg
     1501 gctcatgaac gagagcatac aggggagaag ccctatcaat gcagggattg tgggaaaacc
     1561 ttccaagata agcactgcct taccatccat cagagaatcc acactggaga gaaaccttac
     1621 aaatgtttag agtgtgggaa agctttcagt gggaagtcaa acttgaccaa tcatcgaaga
     1681 attcacactg gagagaagcc tcacaaatgt gaggtatgtg gaatggcctt ccatcatagt
     1741 tcagtcctga ggcagcacaa aagaatccac actggtgaga agccatacac ctgcagtgag
     1801 tgtggcacgt ccttccgtca gggctcagct ctgattggac ataagcgagt tcatactggg
     1861 gagaaacctt atgaatgtga ggaatgtgga aaagctttta gagtgagctc aaatcttact
     1921 ggacataaga aaagaaaaca tcaagtatgg agtacccatg aacttgatgg gagtaggaaa
     1981 tccctctctc cagtgactgt ttctcagacc tcagtagtca gtattttgac cagtgccttg
     2041 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     2101 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     2161 tatatatctt gtggaaagga cgagtataca aacaccttca ccacgga
//
