LOCUS       sequence-286290-        1922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3249
FEATURES             Location/Qualifiers
     source          1..1922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1754..1778)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1786..1827
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgcctcaggt gacatttaat
       61 gatgtggcta tagacttcac tcatgaagag tggggatggc tcagttctgc tcagagggac
      121 ttatacaagg atgtgatggt ccagaattat gagaacctgg tctctgtagc aggtctttct
      181 gtaactaagc catatgtgat cacgttattg gaggatggaa aagagccctg gatgatggag
      241 aaaaaactgt caaaaggtat gattccagat tgggaatcaa gatgggaaaa caaggaatta
      301 tcaacaaaga aggataatta tgatgaagat tcaccccaaa cagtaataat agaaaaagtt
      361 gtaaaacaaa gttatgaatt ttcaaattct aagaagaatt tggaatatat agagaagttg
      421 gaagggaagc atggaagtca ggtagaccat ttcagaccag caattctcac ctctagagaa
      481 agccccactg cagacagtgt ttacaaatac aatatattta gaagcacctt tcattcaaag
      541 tctactcttt ctgaaccaca aaaaatttct gctgaaggga attcacacaa atatgatata
      601 ttaaagaaga acttaccaaa aaagtcagtt ataaaaaatg agaaagtcaa tggtggaaag
      661 aaacttttga attctaataa aagtggggca gccttcagcc agggcaaatc tcttaccctt
      721 ccccagactt gtaatagaga gaaaatctat acatgcagtg aatgtgggaa agcctttggc
      781 aaacagtcaa tcctcaatcg ccactggaga attcatacag gagagaagcc ctatgaatgt
      841 cgtgaatgtg ggaagacttt tagccatggc tcatccctta cacgacatct gataagccat
      901 agtggagaga aaccttacaa atgtattgaa tgtgggaagg cctttagcca tgtctcatca
      961 cttactaacc atcagagcac tcacactgga gagaaaccat atgaatgtat gaactgtgga
     1021 aagtctttta gtcgtgtgtc ccatcttatt gaacatctaa gaattcatac tcaagaaaaa
     1081 ctctatgagt gtcgtatatg tggaaaggcc ttcattcata ggtcatctct cattcaccat
     1141 cagaaaatcc atactggaga gaagccttat gaatgtagag aatgtgggaa agctttctgc
     1201 tgtagctcac accttactcg acatcaaaga attcacacta tggagaaaca atatgaatgc
     1261 aacaaatgtc tgaaagtctt tagtagcctc tcatttcttg ttcagcatca gagtattcat
     1321 actgaagaaa aaccctttga atgtcagaaa tgcaggaaat ccttcaacca gcttgaatca
     1381 ctgaatatgc atttgagaaa tcacattaga ttgaaaccct acgaatgcag tatatgtggg
     1441 aaagccttta gtcataggtc atccctgctt caacatcaca gaattcatac tggagagaaa
     1501 ccttatgaat gtattaaatg tgggaagacc ttcagctgta gttcaaacct taccgtacat
     1561 cagagaattc acactggaga aaagccatat aaatgtaatg agtgtgggaa agcttttagc
     1621 aaaggctcaa atcttactgc ccatcaaaga gtacataatg gagagaaacc caatagtgtg
     1681 gtaagtgtgg aaaagccttt agactatatg aatcactata catgtgagaa atcttacaga
     1741 agagaaactg tattgccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
     1801 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     1861 tcgatttctt ggctttatat atcttgtgga aaggacgagt taaaaaaatt ttagatttcc
     1921 gt
//
