LOCUS       sequence-286293-        1942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3250
FEATURES             Location/Qualifiers
     source          1..1942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1775..1799)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1807..1848
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggctgaaga atcaagaaag
       61 ccttcagccc catccccacc agaccagact cctgaagagg atcttgtaat cgtcaaggta
      121 gaggaggatc atggttggga ccaggaatct agtctgcatg aaagtaaccc tcttggccaa
      181 gaagtgttcc gcctgcgctt caggcagtta cgctaccagg agacactagg accccgagaa
      241 gctctgatcc aactacgggc cctttgccat cagtggctga ggccagattt gaacaccaag
      301 gaacagatcc tggagctgct ggtgctggag cagttcttga ccatcctacc tgaggagctc
      361 cagacactgg ttaaggaaca tcagctagag aacggagagg aggtggtgac cctattagag
      421 gatttggaaa ggcagattga tatactagga cgaccagtct cagctcgcgt acatggacat
      481 agggtactct gggaggaggt agtacattca gcatctgcac cagagcctcc aaatactcag
      541 ctccaatctg aggcaaccca acataaatct ccagtgcccc aagagtcaca agagagagcc
      601 atgtctactt cccagagtcc tactcgttcc cagaaaggaa gttctggaga ccaggaaatg
      661 acagctacac ttctcacagc agggttccag actttggaga agattgaaga catggctgtg
      721 tcccttattc gagaggagtg gcttcttgat ccatcacaga aggatctgtg tagagataac
      781 aggccagaaa atttcagaaa catgttctcc ctgggtggtg agaccaggag tgagaacagg
      841 gaattagctt caaaacaggt aatatctact ggaatccagc cacatggaga gacagctgcc
      901 aaatgcaacg gggatgttat caggggtctt gagcatgaag aagcccgaga ccttctgggc
      961 agattagaga ggcagcgggg aaatcccaca caagagagac gacataaatg tgatgaatgt
     1021 gggaaaagct ttgctcagag ctcaggcctt gttcgccact ggagaatcca cactggggag
     1081 aaaccctatc agtgtaatgt gtgtggtaaa gccttcagtt acaggtcagc ccttctttca
     1141 catcaggata tccacaacaa agtaaaacgc tatcactgta aggagtgtgg caaagccttc
     1201 agtcagaaca caggcctgat tctgcaccag agaatccaca ctggggagaa gccatatcag
     1261 tgcaatcagt gtgggaaggc tttcagtcag agtgcgggcc ttattctgca ccagagaatc
     1321 cacagtggag agagacccta tgaatgtaat gagtgtggga aagctttcag tcatagctca
     1381 cacctcattg gacatcagag aatccacact ggggagaagc cctatgagtg tgatgagtgt
     1441 gggaaaacct tcaggcggag ctcacatctt attggtcatc agaggagcca cactggggag
     1501 aaaccctaca aatgcaatga gtgtgggagg gccttcagtc agaagtcagg ccttattgaa
     1561 catcagagaa tccacactgg agaaagaccc tataaatgta aagaatgtgg gaaagctttc
     1621 aatgggaaca ctggtctcat tcaacacctg agaattcaca caggggagaa gccctaccaa
     1681 tgtaatgagt gtgggaaagc ctttattcag aggtcaagtc tcattcgaca tcagagaatc
     1741 cacagtggtg aaaaatctga atccataagc gttttgccaa ctttcttgta caaagtggtt
     1801 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     1861 agtccgtaac ttgaaagtat ttcgattctt ggctttatat atcttgtgga aaggacgacg
     1921 ggctcgaaac ttatccgtgg ac
//
