LOCUS       sequence-286296-         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3251
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(500..524)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             532..573
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cgcagtctat taacatcacg
       61 gagctgaatc tgccgcagct agaaatgctc aagaaccagc tggaccagga agtggagttc
      121 ttgtccacgt ccattgctca gctcaaagtg gtacagacca agtatgtgga agccaaggac
      181 tgtctgaacg tgctgaacaa gagcaacgag gggaaagaat tactcgtccc actgacgagt
      241 tctatgtatg tccctgggaa gctgcatgat gtggaacacg tgctcatcga tgtgggaact
      301 gggtactatg tagagaagac agctgaggat gccaaggact tcttcaagag gaagatagat
      361 tttctaacca agcagatgga gaaaatccaa ccagctcttc aggagaagca cgccatgaaa
      421 caggccgtca tggaaatgat gagtcagaag attcagcagc tcacagccct gggggcagct
      481 caggctactg ctaaggcctg cccaactttc ttgtacaaag tggttgatat cggtaagcct
      541 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
      601 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaagcatc gatgactcag
      661 tgtaaccc
//
