LOCUS       sequence-286299-        1841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3252
FEATURES             Location/Qualifiers
     source          1..1841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1673..1697)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1705..1746
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg actgcgtgat ctttgaggaa
       61 gtggctgtga acttcacccc agaagagtgg gcattgctgg atcatgctca gaggagcctc
      121 tacagagatg tgatgctgga gacctgcagg aaccttgcct ccttggattg ttacatttat
      181 gttagaacca gtggatcaag ttctcagagg gacgtttttg ggaatggaat atccaatgat
      241 gaagaaattg taaagttcac aggaagtgat tcctggtcta tttttggaga aaattggaga
      301 tttgataaca ctggagatca gcaccaaatc ccacagaggc atctgagaag tcagctgggg
      361 agactctgtg aaagtaatga aggtcatcaa tgcggagaga ccttgagcca gactgcgaac
      421 cttcttgtgc acaagagtta ccctaccgaa gctaaaccct ctgagtgcac taagtgtggc
      481 aaagccttcg agaatcggca gagatctcac actggacaga gaccgtgtaa ggaatgtggg
      541 caagcctgca gctgcctctc ctgccaaagc cctcctatga aaacgcagac tgtagagaaa
      601 ccctgtaatt gccaggactc aaggacagca tctgtgacat acgtgaaaag tctcagcagt
      661 aaaaagtctt atgaatgtca gaaatgtgga aaagctttca tttgtccctc atctttcagg
      721 ggacatgtga atagtcatca tgggcagaaa acccatgcat gtaaagtatg tgggaagacc
      781 tttatgtatt actcctacct tacacggcac gtaagaactc acacaggaga gaaaccctat
      841 gagtgtaagg agtgtgggaa agccttcagc tgtccctcgt actttcgaga acatgtcaga
      901 acacacactg gagagaaacc gtatgaatgt aagcattgtg gaaaatcatt cagctgttac
      961 tcctccttta gagatcacgt gaggacgcac actggagaga aaccctgtca gtgtaaacat
     1021 tgcggaaaag cgttcacttg ttactcgtct cttcgagaac atgggagaac gcacagtgga
     1081 gagaaaccct atgaatgtaa ggaatgcggc aaagccttca ggtacccctc ctctctgcga
     1141 gcacacatga gaatgcacac cggagagaag ccctatgtgt gcaagcagtg tgggaaggcc
     1201 ttcggatgtc ccacttactt tagaagacat gtgaaaacac acagcggggt gaagccctat
     1261 caatgtaaag agtgtgggaa agcctacagt ttttcctcct cccttcgaat ccacgtgagg
     1321 acgcatactg gagagaagcc ctttgagtgt aagcattgtg ggaaagcctt cagctgtcac
     1381 tcctcccttc gagagcatgt gcgaacccac agcggagaga aaccgtacga atgtaatcaa
     1441 tgtgggaaag ccttcagcca cgctcagtac tttcaaaagc atgtgagatc acacagtggg
     1501 gtcaaaccct acgaatgtac tgaatgtggg aaagcctaca gttgttcctc gtcccttcgt
     1561 gtgcacgtga gaacgcacac tggagagaga ccgtatgaat gcaagcagtg tgggaaaacc
     1621 ttcaggtatc tcgcatcgct tcaagcacat gtgagaacac atgctggagc gtgcccaact
     1681 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     1741 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1801 tcttgtggaa aggacgatta gtactatccc tgagcctctg c
//
