LOCUS       sequence-286308-        2339 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3255
FEATURES             Location/Qualifiers
     source          1..2339
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2171..2195)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2203..2244
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc ctgctgatgt gaatttatcc
       61 cagaagcctc aggtcctggg tccagagaag caggatggat cttgcgaggc atcagtgtca
      121 tttgaggacg tgaccgtgga cttcagcagg gaggagtggc agcaactgga ccctgcccag
      181 agatgcctgt accgggatgt gatgctggag ctctatagcc atctcttcgc agtggggtat
      241 cacattccca acccagaggt catcttcaga atgctaaaag aaaaggagcc gcgtgtggag
      301 gaggctgaag tctcacatca gaggtgtcaa gaaagggagt ttgggcttga aatcccacaa
      361 aaggagattt ctaagaaagc ttcatttcaa aaggatatgg taggtgagtt cacaagagat
      421 ggttcatggt gttccatttt agaagaactg aggctggatg ctgaccgcac aaagaaagat
      481 gagcaaaatc aaattcaacc catgagtcac agtgctttct tcaacaagaa aacattgaac
      541 acagaaagca attgtgaata taaggaccct gggaaaatga ttcgcacgag gccccacctt
      601 gcttcttcac agaaacaacc tcagaaatgt tgcttattta cagaaagttt gaagctgaac
      661 ctagaagtga acggtcagaa tgaaagcaat gacacagaac agcttgatga cgttgttggg
      721 tctggtcagc tattcagcca tagctcttct gatgcctgca gcaagaatat tcatacagga
      781 gagacatttt gcaaaggtaa ccagtgtaga aaagtctgtg gccataaaca gtcactcaag
      841 caacatcaaa ttcatactca gaagaaacca gatggatgtt ctgaatgtgg ggggagcttc
      901 acccagaagt cacacctctt tgcccaacag agaattcata gtgtaggaaa cctccatgaa
      961 tgtggcaaat gtggaaaagc cttcatgcca caactaaaac tcagtgtata tctgacagat
     1021 catacaggtg atataccctg tatatgcaag gaatgtggga aggtctttat tcagagatca
     1081 gaattgctta cgcaccagaa aacacacact agaaagaagc cctataaatg ccatgactgt
     1141 ggaaaagcct ttttccagat gttatctctc ttcagacatc agagaactca cagtagagaa
     1201 aaactctatg aatgcagtga atgtggcaaa ggcttctccc aaaactcaac cctcattata
     1261 catcagaaaa ttcatactgg tgagagacag tatgcatgca gtgaatgtgg gaaagccttt
     1321 acccagaagt caacactcag cttgcaccag agaatccact cagggcagaa gtcctatgtg
     1381 tgtatcgaat gcgggcaggc cttcatccag aaggcacacc tgattgtcca tcaaagaagc
     1441 cacacaggag aaaaacctta tcagtgccac aactgtggga aatccttcat ttccaagtca
     1501 cagcttgata tacatcatcg aattcataca ggggagaaac cttatgaatg cagtgactgt
     1561 ggaaaaacct tcacccaaaa gtcacacctg aatatacacc agaaaattca tactggagaa
     1621 agacaccatg tatgcagtga atgcgggaaa gccttcaacc agaagtcaat actcagcatg
     1681 catcagagaa ttcacaccgg agagaagcct tacaaatgca gtgaatgtgg gaaagccttc
     1741 acttctaagt ctcaattcaa agagcatcag cgaattcaca cgggtgagaa accctatgtg
     1801 tgcactgaat gtgggaaggc cttcaacggc aggtcaaatt tccataaaca tcaaataact
     1861 cacactagag agaggccttt tgtctgttac aaatgtggga aggcttttgt ccagaaatca
     1921 gagttgatta cccatcaaag aactcacatg ggagagaaac cctatgaatg ccttgactgt
     1981 gggaaatcgt tcagtaagaa accacaactc aaggtgcatc agcgaattca cacgggagaa
     2041 agaccttatg tgtgttctga atgtggaaag gccttcaaca acaggtcaaa cttcaataaa
     2101 caccaaacaa ctcataccag agacaaatct tacaaatgca gttattctgt gaaaggcttt
     2161 accaagcaat gcccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     2221 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     2281 atttcttggc tttatatatc ttgtggaaag gacgaatcgt ggcgggcttc gccccatgt
//
