LOCUS       sequence-286311-        2045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3256
FEATURES             Location/Qualifiers
     source          1..2045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1877..1901)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1909..1950
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcttcccc gagccccccg
       61 ccggagtcga aggaggagtg ggattatctg gacccagctc agagaagcct gtataaagat
      121 gtcatgatgg agaattatgg aaacctggtc tcactggatg ttttgaacag agataaggat
      181 gaggagccaa ctgtaaaaca agagattgaa gaaattgagg aagaagtgga accacagggt
      241 gtaatagtta caagaatcaa aagtgaaatt gaccaggatc ctatgggtag agaaacattt
      301 gaacttgttg gtaggttaga taaacaaaga gggatcttcc tatgggaaat accaagggaa
      361 tctttgaccc aggaacagag aatgttcaga gaaaacacta acattatccg taaaagacca
      421 aactcagaag agaaatgcca taaatgtgaa gaatgtggaa agggttttgt ccgcaaggcc
      481 catttcattc aacatcaaag ggtccatact ggtgagaaac cttttcagtg caatgaatgt
      541 gggaaaagtt ttagtcgcag ttcatttgtt attgaacatc agagaattca cactggggaa
      601 aggccctatg agtgtaatta ctgtggaaaa acctttagtg tgagctcaac ccttattaga
      661 catcagagaa tccacactgg agaaagaccc tatcagtgta atcagtgtaa acagagcttc
      721 agccagagaa ggagccttgt taaacatcaa aggattcata caggtgagaa accccataaa
      781 tgtagtgact gtgggaaagc cttcagttgg aaatcacacc ttattgagca tcaaagaact
      841 cacactggtg agaaacctta tcactgtacc aaatgtaaga agagctttag tcgaaattca
      901 ttgcttgttg agcatcaaag aattcacact ggggaaagac cccataaatg tggtgaatgt
      961 gggaaagcct ttcgattaag cacatacctt atacaacacc aaaaaattca cactggcgag
     1021 aagccttttc tttgtattga gtgtggaaaa agtttcagtc ggagctcatt ccttattgaa
     1081 catcagagga tccatactgg tgaaagacct tatcagtgca aagagtgtgg gaaaagtttc
     1141 agtcagcttt gcaaccttac tcgtcatcag agaattcaca caggagacaa gccccataaa
     1201 tgtgaggaat gtggaaaagc ctttagtaga agctcaggtc ttattcagca tcagagaatt
     1261 cacaccaggg agaagactta tccatacaat gaaactaagg aaagttttga tccaaattgc
     1321 agtcttgtta tacagcagga agtctaccct aaggagaaat cttataaatg tgatgaatgt
     1381 gggaaaactt ttagtgttag tgctcatctt gtacaacatc aaagaatcca cactggtgaa
     1441 aagccctatc tatgtactgt ctgtgggaaa agcttcagcc ggagctcatt tcttattgaa
     1501 catcagagaa tccacactgg tgagagaccc tatctgtgca gacagtgtgg aaaaagcttt
     1561 agtcagcttt gtaatcttat tcgacatcag ggtgttcaca caggtaataa accccataaa
     1621 tgtgatgaat gtggaaaggc ctttagccgg aactcgggtc ttattcagca tcagagaata
     1681 cacacaggag agaaacctta taagtgtgag aagtgcgaca aaagtttcag tcaacagcgc
     1741 agtcttgtca accatcagaa gatccatgca gaggtgaaaa cccaagaaac ccatgaatgt
     1801 gacgcttgtg gtgaagcctt taattgccgt atttctctta ttcagcatca gaaattgcac
     1861 acagcatgga tgcaattgcc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     1921 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1981 atttcgattt cttggcttta tatatcttgt ggaaaggacg acgtatggtc agatcagccc
     2041 caaga
//
