LOCUS       sequence-286314-        2201 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3257
FEATURES             Location/Qualifiers
     source          1..2201
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2033..2057)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2065..2106
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgcctttaag ggacaaatac
       61 tgtcagactg accaccatca tcacggatgc tgtgaaccag tttatatcct ggaacctgga
      121 gatcctcctt tgttacagca accactacag acatccaaat ctggtattca acaaataatt
      181 gagtgctttc gatcaggaac taaacaactt aagcatattt tattaaaaga tgtggacact
      241 atttttgaat gtaagttatg ccgcagtctc ttcagaggat taccaaattt aattacccat
      301 aaaaaattct actgcccacc aagtctccag atggatgaca accttcctga tgtaaatgat
      361 aaacaaagcc aagccataaa tgatctccta gaagccatat atccaagtgt ggacaaacga
      421 gaatatatta ttaagctaga acccatagaa actaatcaaa atgcagtatt tcaatatatt
      481 tcgaggactg ataatcctat tgaagtcaca gagtcaagca gtactcctga acaaaccgaa
      541 gttcagatac aggaaactag cactgaacag tcaaaaacag taccggttac agatacagag
      601 gtggaaactg tagagccccc tcctgttgag attgttacag atgaagttgc acctacatct
      661 gatgaacaac ctcaggagtc gcaggctgac ttggaaactt ctgacaattc tgattttggt
      721 caccagttga tatgttgtct ttgtagaaaa gaattcaatt ctagacgagg tgttcgccgt
      781 cacattcgaa aagtacacaa gaaaaagatg gaagaactaa aaaagtacat tgaaacacga
      841 aagaatccaa accaatcctc taaaggacgc agtaagaatg ttctagttcc attaagtagg
      901 agttgtccag tatgttgtaa atcatttgct acaaaagcga atgtaaggag gcattttgat
      961 gaagttcata gaggactaag gagggattca attactcctg atatagcaac aaagcctggg
     1021 caacctttgt tcctggattc tatttctcct aaaaaatctt ttaagactcg aaaacaaaag
     1081 tcttcttcaa aggctgaata caatttaact gcatgcaaat gcctcctttg caagaggaaa
     1141 tatagttcac aaataatgct taaaagacat atgcaaattg tccacaagat aactctttct
     1201 ggaacaaact ctaaaagaga aaaaggccct aataatactg ccaacagttc agaaataaaa
     1261 gttaaagttg aaccagcaga ttctgtagaa tcttcacccc cttccattac ccattctcca
     1321 cagaatgaat taaagggaac aaatcattca aatgaaaaaa agaacacacc ggcagcacag
     1381 aaaaataaag ttaaacaaga ctctgaaagc cctaaatcaa ctagtccgtc ggctgcaggt
     1441 ggccagcaaa aaaccagaaa accaaaactt tcagctggct ttgactttaa gcaactttac
     1501 tgtaaacttt gtaaacgtca gtttacttcc aaacagaact tgactaaaca catcgagttg
     1561 cacacagatg gaaataacat ttatgttaaa ttctacaagt gtcctctttg cacttatgaa
     1621 actcgtcgga aacgtgatgt gatacgacat ataactgtgg ttcataaaaa gtcatctcgt
     1681 tatcttggga aaataacagc cagtttagag atcagagcta taaaaaagcc tattgatttt
     1741 gttctaaata aagtggcaaa aagaggccct tcgagggatg aagcaaaaca tagtgattca
     1801 aaacatgatg gcacttctaa ctctcctagt aaaaagtatg aagtagctga cgtcggtatt
     1861 gaagtaaaag tcacaaaaaa cttttctctt cacagatgca ataaatgtgg aaaggcattt
     1921 gccaaaaaga cttaccttga acatcataag aaaactcata aggcaaatgc ttccaattca
     1981 cctgaaggaa acaaaaccaa aggccgaagt acaagatcta aggctcttgt cttgccaact
     2041 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     2101 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2161 tcttgtggaa aggacgaaac tgacacccca ttactacctt t
//
